HAUPTMAN-WOODWARD INSTITUTE STRUCTURAL STUDIES OF MEDICALLY RELATED PROJECTS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。来自Hauptman-Woodward医学研究所的一个合作研究小组将几个晶体结构测定项目整合到一个提案中。该提案包括不同阶段的医学相关项目，需要同步加速器时间来延长分辨率，从非常小的晶体中获取数据，并为结构解决目的进行数据收集，即需要实验室中可用的波长以外的波长用于异常衍射。该研究包括对非核糖体肽合成酶的研究，这是抗生素开发的一个潜在领域，以及参与铁载体生产的蛋白质途径。超高分辨率的数据将收集人类自整合障碍因子以及短链氧化还原酶家族小晶体的数据。还将收集两种膜蛋白COX-2和PIOX的数据，这两种膜蛋白都参与了多不饱和脂肪酸前体的氧化脂素生物合成。通过合作，同步加速器时间和内部专业知识得到了最佳利用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A collaborative group of researchers from the Hauptman-Woodward Medical Research Institute have consolidated several crystallographic structure determination projects into a single proposal. The proposal includes medically related projects at various stages requiring synchrotron time to extend resolution, get data from very small crystals and perform data collection for structural solution purposes i.e. requiring wavelengths other than that available in the laboratory for anomalous diffraction. The research includes the study of Non-Ribosomal Peptide Synthetases, a potential area for antibiotic development, and the pathways of proteins involved in siderophore production. Ultra-high resolution data will be collected on human barrier-to-autointegration factor as well as data from small crystals of the short chain oxidoreductase family. Data will also be collected on two membrane proteins, COX-2 and PIOX, both of which are involved in oxylipin biosynthesis from polyunsaturated fatty acid precursors. By collaboration best use is made of the synchrotron time and in-house expertise.