Implantable Glucose Microsensor for Wireless Telemetry

这项提议目标是开发一种集成的、可植入的 微型遥测血糖传感器。这种装置是基于对葡萄糖敏感的 微机械加工条件下水凝胶的溶胀和收缩 微腔，但通过坚硬的、多孔的、 尺寸选择膜。膨胀和收缩的力量是作为一种 葡萄糖与侧链苯基硼酸络合的直接结果 水凝胶上的基团，没有葡萄糖氧化酶等酶的介导 或者己糖激酶。该设备不需要植入电池 经皮穿刺线。膨胀或收缩的力量，响应于 葡萄糖浓度的变化，撞击到微型电容器的极板上， 改变其间隙大小和电容，导致电磁特性发生变化 密封微细加工电路的谐振频率，它可以 通过手持式或手表大小的审问单元进行远程检测。在 目前的建议我们将研究水凝胶组成的影响，以及 设备组件的材料和几何属性，对性能的影响 微型传感器然后我们将为体外和体内构建一个原型设备 测试。体外实验将探索设备正确响应的能力。 葡萄糖浓度的变化，并评估其他化学物质的影响 物种，如小糖类和血浆蛋白。在活体实验中， 在大鼠身上进行实验，将重点放在可植入传感器的忠实能力上 监测血糖浓度的一系列阶梯变化。

The objective of this proposal is develop an integrated, implantable, microtelemetric glucose sensor. This device is based on the glucose-sensitive swelling and shrinking of hydrogels that are confined within micromachined microcavities, but are in communication with body fluids through a rigid, porous, size-selective membrane. Swelling and shrinking forces are developed as a direct consequence of glucose complexation with pendant phenylboronic acid groups on the hydrogel, without mediation by enzymes such as glucose oxidase or hexokinase. This device does not require implanted batteries of transcutaneous wires. Swelling or shrinking forces, generated in response to changes in glucose concentration, impinge on a plate of a microcapacitor, altering its gap size and capacitance, leading to a change in the electromagnetic resonant frequency of a hermetically sealed, microfabricated circuit, which can be remotely sensed by a handheld or wristwatch-sized interrogating unit. In the present proposal we will investigate the effects of hydrogel composition, and material and geometric properties of the device components, on performance of the microsensorWe will then construct a prototype device for in vitro and in vivo testing. In vitro experiments will probe the devices¿ ability to respond properly to changes in glucose concentration, and assess the effect of other chemical species, such as small saccharides and plasma proteins. In vivo experiments, performed in rats, will focus on the ability of implantable sensors to faithfully monitor series of step changes in blood glucose concentration.