A Novel Method of Ion Fragmentation in Tandem Mass Spectrometry

描述(由申请人提供)：拟议研究的主要目标是开发一种用于高通量蛋白质组应用的串联式正交式加速飞行时间(TOF)质谱仪，具有增强的MS/MS能力。这将通过建立一个能够在两种不同的MS/MS模式下运行的双串联仪器来实现。除了传统的碰撞诱导解离(CID)，该仪器还将采用一种基于多肽离子与亚稳原子或分子流动相互作用的新型碎裂技术。这种新的裂解技术将切割不同于CID的多肽主链，对多肽组成的依赖性更小，并保留不稳定的翻译后修饰。将这两种技术整合到一台高性能Q-TOF质谱仪中，就可以将这些仪器用于高通量蛋白质组学应用。蛋白质组学通过对细胞或组织中表达的蛋白质进行系统分析来研究生物过程。它在现代生命科学、药物开发和临床应用中发挥着重要作用。我们提出了一种新的高通量技术，以提高利用串联质谱学进行蛋白质鉴定和结构鉴定的效率。

DESCRIPTION (provided by applicant): The primary objective of the proposed research is the development of a tandem orthogonal acceleration time-of-flight (TOF) mass spectrometer for high throughput proteomics applications with enhanced MS/MS capabilities. This will be achieved by building a dual tandem instrument capable of operating in two different MS/MS modes. Alongside with traditional collision induced dissociation (CID), this instrument will feature a novel fragmentation technique based on the interaction of peptide ions with a flow of metastable atoms or molecules. This new fragmentation technique will cleave different peptide backbone bonds than CID with less dependence on peptide composition and preserve labile posttranslational modifications. Incorporating both techniques in one high performance Q-TOF mass spectrometer will allow using these instruments for high throughput proteomics applications. Proteomics studies biological processes by the systematic analysis of the proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new high throughput technology for increasing the efficiency of protein identification and structure elucidation using tandem mass spectrometry.