Capsular Assemblies Driven by the Hydrophobic Effect

项目摘要 这项提议的长期目标是：1)了解分子结构和疏水性 效果结合以促进纳米胶囊在水溶液中的组装；2)利用这些信息来 两人都了解了四元蛋白质的结构，并设计出了自组装纳米胶囊， 起药物输送系统的作用。 这项提议的目的是合成和分析可组装成 纳米级的胶囊，在这样做的过程中，客体分子被困在其中空的内部。四个空洞 空腔形状和大小不同，以及促进组装的疏水表面的面积将是 合成的。每一种都可以用一层外层的功能来调节它们的组装 属性。将这些变量结合在一起，就会产生数百个同质和异质胶囊。胶囊 编队将作为客人的一项职能进行检查，以确定他们如何影响集会。此外, 我们将研究胶囊是如何动态稳定正常活性化合物的。特别感兴趣的 在这方面，将对潜在的抗疟疾药物进行分析，这些药物太不稳定或无法溶解到 个人展示活动。系统的分析将用核磁进行 核磁共振光谱、等温滴定量热法和荧光光谱。 相关性 新药物输送系统的发展为攻击疾病状态提供了新的途径。在……里面 模仿自然界最有效的药物传递系统--病毒--这项提案概述了研究 研究纳米胶囊的形成和性质。到目前为止，人们对纳米胶囊是如何 在水中组装。因此，这些研究将为这一新领域提供有价值的信息。 研究。

Project Summary The long-term objectives of this proposal are: 1) to learn how molecular structure and the hydrophobic effect combine to promote the assembly of nano-capsules in aqueous solution; 2) to use this information to both gain an understanding of quaternary protein structure, and devise self-assembling nano-capsules that function as drug delivery systems. The aim of this proposal is the synthesis and analysis of water-soluble cavitands that assemble into nano-scale capsules, and in doing so entrap guest molecules within their hollow interiors. Four cavitands that differ in cavity shape and size, and the area of hydrophobic surface that promotes assembly, will be synthesized. Each can be "coated" with an external layer of functionality that modulates their assembly properties. In combination these variables engenders hundreds of homo- and hetero-capsules. Capsule formation will be examined as a function of guest to determine how they influence assembly. In addition, we will examine how the capsules kinetically stabilize normally reactive compounds. Of particular interest in this regard will be the analysis of potential anti-malarial drugs that are too unstable or insoluble to individually demonstrate activity. Analysis of the systems will be carried out with Nuclear Magnetic Resonance (NMR) spectroscopy, Isothermal Titration Calorimetry (ITC), and fluorescence spectroscopy. Relevance The development of new drug delivery systems allows new avenues for attacking disease states. In mimicry of Nature's most efficient drug delivery systems- viruses - this proposal outlines investigations into the formation and properties of nano-capsules. To date, little is known about how nano-capsules assemble in water. Hence, these studies will provide valuable information pertaining to this novel field of research.