EFFECT OF N-3 FATTY ACIDS ON LIVER FAT CONTENT

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项研究有两个具体目的：1)确定胰岛素抵抗与肝脏脂肪含量是否存在关联；2)确定n-3多不饱和脂肪酸是否能减少肝脏中的脂肪量。肥胖常常伴随非酒精性脂肪肝(NAFL)。然而，并不是所有肥胖的受试者都会增加肝脏脂肪。在第一个目标中，将确定患有NAFL的肥胖者是否存在肌肉和脂肪组织的胰岛素抵抗。肌肉的胰岛素抵抗将通过量化胰岛素介导的葡萄糖摄取来研究，脂肪组织的胰岛素抵抗将通过非酯化游离脂肪酸(NEDA)的代谢参数来研究。N-3多不饱和脂肪酸[二十碳五酸(EPA)和二十二碳六烯酸(DHA)]对脂肪酸和甘油三酯代谢有多种影响，人体服用这些脂肪酸可降低血浆甘油三酯水平。在这个项目中，我们将确定鱼油(EPA和DHA的混合物)在降低NAFL肝脏脂肪含量方面的治疗效果。还将测量骨骼肌和心脏的脂肪含量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study has two specific aims: 1) to determine whether there is an association between insulin resistance and liver fat content and 2) to determine whether n-3 polyunsaturated fatty acids reduce the amount of fat in the liver. Obesity is frequently accompanied by non-alcoholic fatty liver (NAFL). However, not all obese subjects have increased hepatic fat. In the first aim, it will be determined if obese subjects with NAFL have insulin resistance of muscle and adipose tissue. Insulin resistance of muscle will be studied by quantitation of insulin-mediated uptake of glucose, insulin resistance of adipose tissue will be investigated through parameters of metabolism of nonesterified free fatty acids (NEDA). N-3 polyunsaturated fatty acids [eicosopentanoic acid (EPA) and docosahexaenoic acid (DHA)] have multiple effects on fatty acid and triglyceride metabolism and administration of these fatty acids to humans results in a reduction of plasma triglyceride levels. In this projects we will determine the therapeutic efficacy of fish oils (a mixture of EPA and DHA) at reducing hepatic fat content in NAFL. Measurements also will be made of fat content of skeletal muscle and heart.