DIETARY PROTEIN INTAKE IN MAINTENANCE OF HEMODIALYSIS PATIENTS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。本项目由两项研究组成。研究1包括9至13名维持性血液透析(MHD)患者，他们临床稳定，将接受氮平衡研究，同时每人摄入17天的膳食蛋白质摄入量，提供0.60、0.80、1.00、1.15和1.30克蛋白质/公斤/天。13C-亮氨酸周转研究13C-尿素动力学研究和血浆氨基酸、透析液氨基酸、多肽和蛋白质的测量，肌肉活检以测量IGF-I、IGF-II、IGF受体、肌肉生长抑素和IGF结合蛋白的蛋白和基因表达，以及身体组成(人体测量和双X射线波顿吸收法(DXA))。在研究2中，70-80名MHD门诊患者将被随机分配到1.00或1.30g/kg/天的DPI，为期5个月。总蛋白质将通过测量总氮和总钾来评估。身体成分的其他成分将使用人体测量、DXA、BIA和近红外电抗进行评估。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is composed of two studies. Study 1 consists of 9 to 13 maintenance hemodialysis (MHD) patients who are clinically stable and will undergo nitrogen balance studies while they ingest, for 17 days each, diet protein intakes providing 0.60, 0.80, 1.00, 1.15 and 1.30 g protein/kg/day. 13 C-leucine turnover studies 13C-urea kinetic studies and measurements of plasma amino acides, dialysate amino acids, peptides and proteins, muscle biopsies for measurement of protein and gene expression for IGF-I, IGF-II, IGF-receptor, myostatin, and IGF-binding proteins and body composition (anthropometry and dual x-ray pohton absorptiometry (DXA)) will be measured. In Study 2, 70-80 MHD outpatients will be randomly assigned to a DPI of 1.00 or 1.30 g/kg/day for five months each. Total body protein will be assessed by measurements of total body nitrogen and total body potassium. Other components of body composition will be assessed using anthropometry, DXA, BIA and near infrared interactance.