CONSENT TO DRAW BLOOD FOR THE EVALUATION OF GHRELIN

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。非天然的五肽GHRPs出现于1976年。1984年在大鼠实验中发现GHRP-GHRH可协同释放生长激素，表明二者具有互补和独立的作用。GHRP被假设反映了一种新的下丘脑激素的活性。到1989年，GHRP- ghs受体在人类中的释放是显著的，到1996年，GHRP- ghs受体被克隆。天然激素grehlin已被Kojima等人分离出来。其化学性质和主要的起源解剖部位是新颖的，因为它是一种ser3 -辛酸化的28个氨基酸肽，似乎起源于胃，但也存在于下丘脑锐核的腹侧边界。我们在大鼠实验中的初步结果支持天然ghrelin与非天然GHRP具有相同的生长激素释放活性。在大鼠、狗和人的体内外研究中，已经提出了以下假设：1)ghrelin是生理性内源性GHRP激素，2)GHRP-2和ghrelin本质上具有相同的生物学作用，3)天然GHRP， ghrelin缺乏是导致一些正常老年人GH分泌减少的原因。我们现在有方法来测量胃饥饿素的基础水平。我们已经使用了商业RIA试剂盒，并试图在我们的实验室建立一个RIA来测定血浆/血清中的胃饥饿素水平。这将使我们能够确定不同个体在一天的不同时间，餐前和餐后的循环胃饥饿素水平，以便评估受试者之间这种肽水平的准确性和差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The unnatural pentapeptide GHRPs appeared in 1976. GHRP-GHRH was found to synergistically release GH in rats in 1984, indicating the peptides' complementary and independent actions. GHRP was hypothesized to reflect the activity of a new hypothalmic hormone. By 1989, the GHRP GH release in humans was notable, and, by 1996, the GHRP-GHS receptor was cloned. The natural hormone, grehlin, has been isolated by Kojima et al. The chemistry and the major anatomical site of origin are novel since it is a Ser3-octanolyated 28 amino acid peptide that appears to originate in the stomach but also is present in the ventral lateral border of the hypothalamic acuate nucleus. Our preliminary results in rats support that natural ghrelin has the same GH releasing activity as the biological actions of unnatural GHRP. In vitro and in vivo studies in rats, dogs, and humans have been proposed to pursue the hypothesis that 1) ghrelin is the physiological endogenous GHRP hormone, 2) GHRP-2 and ghrelin essentially have the same biological actions, and 3) natural GHRP, ghrelin, deficiency is responsible for the decreased GH secretion of some normal older humans. We now have methods to measure the basal levels of ghrelin. We have used commercial RIA kits and are attempting to establishan RIA in our laboratory to determine ghrelin levels in plasma/serum. This will enable us to ascertain the levels of circulating ghrelin of different individuals at various times of the day, before and after meals, in order to assess the precision and differences in the levels of this peptide between subjects.