A laser cutting and laser capture microscope for plant biology

多细胞生物体具有包含各种细胞类型的组织。每种细胞类型都有区别，以执行定义的功能。我们对发生在特定细胞中的过程感兴趣。其目的是确定机制，允许差异基因表达在特定的细胞中，这种差异表达在这些细胞中的后果。例如，申请人1旨在鉴定细胞类型之间启动子元件甲基化的差异，申请人3-5希望确定来自特定细胞类型的转录物丰度，然后使用该信息产生发育和昼夜节律网络，或理解对病原体攻击或细胞信号传导的响应。在基因表达分析之前，通过LCM收集了少至10个植物细胞，因此所有提出的工作都是可行的，我们应该在每个领域取得重大进展。

Mutlicellular organisms possess tissues containing various cell types. Each cell type differentiates to perform defined functions. We are interested in processes that occur in specific cells. The aim is to identify mechanisms allowing differential gene expression in particular cells, and the consequences of this differential expression in those cells. For example, Applicant 1 aims to identify differences in methylation of promoter elements between cell-types, applicants 3-5 wish to determine transcript abundance from specific cell-types, and then use that information to generate developmental and circadian networks, or understand responses to pathogen attack or cell signalling. As few as ten plant cells have been collected via LCM prior to analysis of gene expression, and so all the work proposed is feasible and we should make significant advances in each of our fields.