RACIAL DIFFERENCES IN CIRCULATING SEX STEROIDS: MECHANISMS AND EFFECT ON BONE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。本研究的目的是描述与白色女性相比，黑人女性在整个月经周期中的类固醇水平特征，并监测肥胖和种族对性类固醇水平和骨矿物质密度和结构的影响。似乎有强有力的和越来越多的证据表明，有根本性的差异，在循环性类固醇水平，这有助于众多的最终器官的变化，在黑人。我们建议更详细地记录这些，首先考虑种族群体之间肥胖的差异。此外，迄今为止，除了描述性研究外，没有其他研究，对潜在机制的探索很少。 在本研究中，将对200只正常骑自行车的黑人和白色血统雌性动物进行研究。我们将深入研究月经周期，每天收集尿液，并为一个周期抽血和卵泡发育的超声文件。我们将描述月经周期过程中循环性类固醇的特征。此外，我们将检查身体组成和骨密度和建筑在这一组。我们还将收集血清，以便我们可以在该队列中观察胰岛素抵抗，心血管风险，DNA和血清蛋白质组学。我们建议建立规范性数据的性激素水平在黑人妇女的历史规律的月经周期，并比较这些与他们的白色同行。将在体重、身高和BMI正常的女性中研究连续三个月经周期。这些数据将作为我们的主要成果。我们还建议测量肥胖黑人和白色女性的性激素水平，收集血清供将来使用，以便我们可以观察胰岛素抵抗、心血管风险、DNA和血清蛋白质组学，并计算所有组的骨矿物质密度和结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to characterize the steroid level profile throughout the menstrual cycle in Black females compared to White females, and to monitor the effects of obesity and race on sex steroid levels and bone mineral density and architecture. There appears to be strong and increasing evidence that there are fundamental differences in circulating sex steroid levels, which contribute to a multitude of end organ changes in Blacks. We propose to document these in greater detail, above all accounting for differences in obesity between racial groups. Additionally there has been nothing but descriptive studies to date, and little exploration of potential mechanisms. In this study 200 normally cycling females of Black and White origin will be studied. We will intensively study the menstrual cycle with daily urinary collections, and for one cycle blood draws and ultrasound documentation of follicular development. We will characterize circulating sex steroid during the course of the menstrual cycle. Additionally we will examine body composition and bone mineral density and architecture in this group. We will also collect serum so that we can look at insulin resistance, cardiovascular risks, DNA, and serum for proteomics in this cohort. We propose to establish normative data for sex hormone levels in Black women with a history of regular menstrual cycles and compare these with their White counterparts. Three consecutive menstrual cycles will be studied in women who are of normal body weight, height, and BMI. These data will serve as our primary outcome. We also propose to measure sex hormone levels in obese Black and White women, collect serum for the future so we can look at insulin resistance, cardiovascular risks, DNA and serum proteomics, and in all groups bone mineral density and architecture will be calculated.