FLOURESCENT PROBES & IMAGING FOR NETWORKS & PATHWAYS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。描述（由申请人提供）：我们建议建立一个全国可见的响应中心，专注于荧光探针和成像技术，用于在活细胞中以真实的时间和高分辨率研究途径和网络。拟议的中心将结合匹兹堡现有的两个中心的经验和基础设施。该中心的研究部分将创建一个强大的细胞内荧光标记和生物传感器工具箱，可用于研究许多（如果不是全部）活细胞通路和网络中的蛋白质。强荧光探针开发计划融合了遗传学、蛋白质结构、核酸结构和荧光染料化学。我们建议产生的探针将是可遗传表达的，这样外源大分子就不必被运送到活细胞中进行研究。除了技术开发核心外，四个一流的“驾驶生物学项目”将成为整个中心计划的一部分。这些项目位于匹兹堡大学、卡内基梅隆大学、斯坦福大学和伯克利大学。他们的责任是协助新中心确定最佳的开发技术，并就技术的实用性提供重要的反馈。成像和信息学技术与新的荧光探针技术相结合，这些组合工具将帮助细胞生物学家获得有关活细胞中通路的大量空间和时间信息。提案中包括使荧光探针和成像技术稳健的计划，以便它们可以提供给学术研究，药物发现和生物技术中的广泛细胞生物学家，并传播这些技术并提供培训

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We propose to form a nationally visible and responsive center focused on fluorescent probe and imaging technologies for investigating pathways and networks in real time and at high resolution in living cells. The proposed Center will be formed by combining the experience and infrastructure of two already existing Centers in Pittsburgh. The research component of the Center will create a powerful toolbox of intracellular fluorescent labels and biosensors that can be used to study many, if not all, the proteins in pathways and networks of living cells. The strong fluorescent probe development program blends genetics, protein structure, nucleic acid structure and fluorescent dye chemistry. The probes we propose to generate will be genetically expressible so that exogenous macromolecules will not have to be transported into the living cells to be studied. Four superb "Driving Biology Projects" are to be part of the overall Center program in addition to the Technology Development Core. These projects are located at the University of Pittsburgh, Carnegie Mellon, Stanford and Berkeley. Their responsibility is to assist the new center in identifying the optimal technologies for development and for providing crucial feedback regarding the utility of the technologies. Imaging and informatics technologies are integrated with the new fluorescent probe technologies so that these combined tools will help cell biologists obtain large amounts of spatial and temporal information about pathways in living cells. Included in the proposal are programs for making the fluorescent probe and imaging technologies robust so that they can be provided to a wide range of cell biologists in academic research, pharmaceutical drug discovery, and biotechnology and to disseminate these technologies and provide training