EARLY MARKERS FOR BREAST CANCER RISK IN NIPPLE ASPIRATE FLUID

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。背景简介：近年来，乳头吸出液(NaF)被认为是乳腺癌危险标志物的潜在来源。NaF的主要成分是蛋白质，包括各种激素调节蛋白，以及众所周知的癌症标志物，如前列腺特异性抗原(PSA)和癌胚抗原(CEA)。然而，目前还没有确定与乳腺癌风险相关的特定NaF蛋白图谱的方法。具体目标和程序：NaF的蛋白质图谱将通过最先进的技术进行分析，包括二维凝胶电泳(2D Gel)和基质辅助激光解吸/电离飞行时间质谱仪(MALDI-TOF MS)。这两种技术的结合使用使我们能够研究低风险健康女性的全球蛋白质图谱，这些方法既省时又省钱，很容易适应临床应用。实验设计：在治疗前，对40名无乳腺癌家族史的健康女性、40名一级亲属确诊为乳腺癌的健康女性和40名新诊断为乳腺癌(包括单侧乳腺癌)的女性进行NaF蛋白图谱的研究和比较。意义：本研究的最终目的是发现和分类NaF蛋白，这些蛋白是乳腺癌风险的早期标志。在NAF中识别乳腺癌危险标记物可能会使乳腺癌的检测发生革命性的变化，因为它可以无限次、无创地进行，从而为我们提供了一种前瞻性地跟踪普通人群和更频繁地跟踪高危人群的工具。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brief Background: In recent years, nipple aspirate fluid (NAF) has been considered as a potential source for breast cancer risk markers. The main components of NAF are proteins, including various hormone-regulated proteins, and generally known cancer markers such as prostate specific antigen (PSA) and carcinoembryonic antigen (CEA). Currently, however, there is no established way to identify the specific NAF protein profiles associated with breast cancer risk. Specific Aims and Procedures: Protein profiles of NAF will be analyzed by state-of-the-art techniques, including two-dimensional gel electrophoresis (2-D gel) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The combined use of these two technologies has enabled us to study global protein profiles in low risk healthy women and these methods are time- and cost-efficient and could be easily adapted for clinical applications. Experimental Design: The NAF protein profiles will be studied and compared among three groups of women: 40 healthy women with no family history of breast cancer, 40 healthy women with first-degree relatives diagnosed with breast cancer, and 40 women with newly diagnosed breast cancer, including those with unilateral breast cancer, before treatment. Significance: The ultimate purpose of this study is to discover and catalog NAF proteins that are early markers for breast cancer risk. Identification of breast cancer risk marker in NAF may revolutionize breast cancer detection because it can be performed an unlimited number of times and non-invasively and thus provides us a tool to follow prospectively the general population and follow more frequently the higher risk populations.