HRT AND PROTEIN EXPRESSION IN POSTMENOPAUSAL WOMEN

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。2.1研究假设：对健康绝经后妇女开始HRT前后的血清和血浆进行蛋白质组学评估，将检验外源性HRT给药导致蛋白质差异表达的假设。还将对仅接受雌激素治疗组与联合（CEE + MPA） HRT治疗组之间蛋白质表达差异的假设进行测试。2.2具体目的“确定健康绝经后妇女开始激素替代治疗前后血清和血浆蛋白目录。”使用差异分析来确定血清和血浆蛋白表达作为HRT暴露的功能，通过比较：o在妇女组内，开始HRT之前和一个月后，o在两组妇女之间，通过使用雌激素单独或联合HRT方案分层。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 2.1 Study Hypotheses Proteomic evaluation of the serum and plasma of healthy postmenopausal women before and after initiating HRT will test the hypothesis that proteins are differentially expressed as a result of exogenous HRT administration. The hypothesis that proteins are differentially expressed between groups treated with estrogen-only versus those treated with combination (CEE + MPA) HRT will also be tested. 2.2 Specific Aims " To define the protein catalogue of serum and plasma of healthy postmenopausal women before and after initiation of HRT. " To use differential analysis to identify serum and plasma proteins expressed as a function of exposure to HRT, by comparing: o Within the group of women, before and one month after initiation of HRT, and o Between two groups of women, stratified by use of estrogen-only versus combination HRT regimens.