ESSENTIAL AND NON-ESSENTIAL ELEMENT BIOAVAILABILITY FROM SUBSISTENCE FOODS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。野生动物毒理学实验室购置并安装了主要设备，并培训了两名技术员（Knott女士和Swor女士）和两名学生（Kirk女士和Moses女士）使用Perkin Elmer AA 800原子吸收分光光度计、布鲁克斯兰德汞分析仪（总汞和单甲基汞）以及与健康评估（影响）有关的其他设备。在Gerald Bratton博士的指导下，与德克萨斯A&M大学（学院站）的微量元素实验室建立了QA/QC程序，并对未来食品（有蹄类动物、海豹等）的大量样品进行了检测。的北极狐（Alopex lagopus）饲养试验的必需和非必需元素进行了分析。Sara Moses女士正在领导2005年1月启动的质量保证/质量控制工作。海洋哺乳动物组织分析将是国家标准与技术研究所（NIST）QA/QC计划的一部分。在实验室经理（Swor女士）和PI的指导下，实验室所有人员正在建立基本健康和免疫状态检测。这项研究的北极狐捕获将于2005年6月进行，饲养试验将于今年夏天开始。我们将确定的基本（铜，硒，锌）和非必需（镉，汞）元素和稳定同位素的碳和氮富集的北极狐从这些选择的生存食品项目的一般生物利用度。从长远来看，我们将确定北极狐模型是否适合实验室研究，以解决与阿拉斯加北极人类和野生动物食物网中的污染物和感染因子相关的免疫，生殖和疾病传播问题。实验室继续招聘博士后助理。一个前景是博士。该计划在威斯康星州麦迪逊大学，并可能提供明年（2006年）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Wildlife Toxicology Laboratory has acquired and installed major equipment and trained two technicians (Ms. Knott and Ms. Swor) and two students (Ms. Kirk and Ms. Moses) on the use of the Perkin Elmer AA800 Atomic Absorption Spectrophotometer, Brooks Rand Mercury Analyzer (total and monomethyl) and other equipment associated with the health assessment (impacts). A QA/QC program was established with the Trace Elements Laboratory at Texas A&M University (College Station) under the guidance of Dr. Gerald Bratton, and numerous samples of future food items (ungulates, seals, etc.) for the arctic fox (Alopex lagopus) feeding trial were analyzed for essential and non-essential elements. Ms. Sara Moses is leading this QA/QC effort that was initiated January 2005. The marine mammal tissue analyses will be a part of a QA/QC program with the National Institute of Standards and Technology (NIST). Basic health and immune status assays are being established by all in the laboratory under the guidance of the Laboratory Manager (Ms. Swor) and the PI. Capture of arctic foxes for this study will occur June 2005 and the feeding trial initiated this summer. We will determine the general bioavailability of essential (Cu, Se, Zn) and non-essential (Cd, Hg) elements and stable isotopes of carbon and nitrogen enrichment to the arctic fox from these select subsistence food items. In the longer term we will determine the suitability of the arctic fox model for laboratory studies to address immune, reproductive and disease transmission issues related to contaminants and infectious agents in the arctic Alaska human and wildlife food web. The laboratory continues to recruit for a Postdoctoral assistant. One prospect remains in a Ph.D. program at the University of Wisconsin Madison and could be available next year (2006).