INBRE CENTRAL INSTRUMENTATION CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。特拉华INBRE年度进展报告（APR） 2006拨款号2P20RR016472-2006共享研究仪器核心特拉华INBRE共享研究仪器核心提供位于特拉华生物技术研究所和特拉华大学几个部门的共享的、主要集中的仪器，供全州网络的研究人员使用。目前，每个学术伙伴机构都有独立的、较小的生物医学研究和培训实验室。研究仪器中心由一批高素质的科研人员管理。随着整个网络的研究，对这些设施的利用不断增长。在INBRE项目的第二年，建立了评估机制来跟踪这些共享设施的使用和增长。各核心中心主任定期召开会议，分享共同的业务战略。核心中心的费用减免部分由INBRE资助，为教师和学生研究人员提供支持，使他们能够访问中心，建立基线数据集，用于资助开发或启动学生项目。特拉华INBRE支持为教师、研究生和其他研究人员举办研讨会，向他们介绍网络内的可用功能。核心中心的持续重点是实施最佳管理做法，以确保这些设施的有效运作和长期可持续性。特拉华州INBRE的研究仪器中心包括：仪器中心-核心中心工作人员-2005用户群[教师组#]生物成像Kirk Czymmek博士，Elizabeth Adams博士，Deborah Powell， BS 48生物信息学Douglas O¿Neal博士，MS, Leo Genyuk， MS, Eric Garrison， MS， 17 DNA测序和基因分型Brewster Kingham， MS 42质谱John Dykins， MS 6微阵列Dianne York， MS 11核磁共振Steve Bai博士，Heather Egolf-Fox， BS 10植物生长室Robert Pekala，仪器核心在二年级的12项成就包括：1)生物医学核心中心设施的全州研究基础设施，配备了最先进的仪器和专业人员。2)一个新的核心中心门户网站（www.dbi.udel.edu/coreinstrumentation.html）现在可供INBRE网络的教师和学生查找研究资源。核心中心门户网站描述了可用的仪器，列出了核心中心主任和工作人员的联系信息，并提供了服务的定价信息。特拉华州立大学和DBI的几个核心中心主任自愿花时间帮助教授特拉华州立大学生物技术项目的一门关键课程。在2005-2006学年，五名生物科技大四学生需要选修仪器课程，以符合2006年5月毕业的要求。Microarray核心中心经理Dianne York担任课程协调员。她制定了教学大纲，并与其他核心主任一起安排了讲座和实验室工作的时间表，并协调了考试的内容和管理。这个独特的课程以研讨会的形式组织。4) 2005年11月11日在DBI举行了核心中心开放日。开放日活动包括所有核心中心主任的演讲，随后是参观和实验室演示。来自整个网络的30多名与会者参加了此次活动。根据积极的反馈，这个活动现在将每年举行一次。5) 2006年1月18日至19日，微阵列核心中心举办了一个微阵列打印工作坊。研讨会全部预定，来自几个合作机构的8名教师和学生研究人员参加了研讨会。根据非常积极的评价，一个后续讲习班正在规划阶段。6) 2005年5月，DBI举办了一场介绍三大基因组浏览器的会议，由OpenHelix介绍。为期一天的研讨会提供了概述和实践环节，主要介绍了Ensembl基因组浏览器、UCSC基因组浏览器和NCBI地图查看器。7)生物成像核心中心举办了几次培训研讨会，介绍了新的成像仪器，包括新的生物力显微镜和用于inbre资助的激光捕获显微解剖仪器的新的激光镊子选项。8)生物信息学中心正在最后确定一个软件门户，以便INBRE网络的所有成员都能方便地使用大量的计算资源。该门户将允许用户向计算服务器队列提交新的计算作业，并改善对广泛的生命科学和生物医学软件程序的访问。9)核心中心主任每两个月召开一次会议，讨论日程安排、计费、广告和联合研讨会等常见问题的最佳实践。10)通过参加区域和国家会议以及内部讲习班，继续对科学核心中心工作人员进行专业教育。11)核心中心费用减免计划由INBRE部分支持，为新研究人员提供免费访问核心中心的发展项目，从而为未来的拨款提案创建基线数据。12) INBRE网络中的合作机构在二年级增加了以下研究仪器：a)特拉华大学增加了生物成像和生物信息学中心的能力，包括：用于FESEM样品制备的临界点干燥机。该系统提供了在FESEM上制备生物样品进行成像的能力，并通过干燥过程保持样品的细胞完整性。该系统极大地扩展了我们FESEM的用户基础。太阳生物狼计算集群的额外内存模块（96 gb）。这次升级提高了集群的性能，具有更高的基因组搜索吞吐量。b)特拉华州立大学通过获得Internet 2联盟的会员资格作为赞助参与者，完成了对校园范围IT结构的重大升级安装。通过DSU和UD之间的密切合作，这种能力将为DSU校园内的部门和研究设施提供高速连接。c)特拉华技术与社区学院购买了设备，以改善联合健康计划和科学课程的教学。这些设备包括：跑步机，通风分析设备，物理实验室设备，显微镜，天平，计算机，化学和生物实验室设备d)韦斯利学院购买的仪器，以扩大本科生的研究经验。该仪器将提供以前不可用的分析技术，以及培训和演示工具，以向卫斯理内部更广泛的受众提供这些信息。这包括对生物信息学系统实验室的升级和软件更新，为新教员设置实验室，以及改善与校园的互联网连接。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Delaware INBRE Annual Progress Report (APR) 2006 Grant No. 2P20RR016472-2006 Shared Research Instrumentation Core The Delaware INBRE Shared Research Instrumentation Core provides shared, largely centralized instrumentation located at the Delaware Biotechnology Institute and at several departments of the University of Delaware to researchers throughout the statewide network. Individual, smaller biomedical research and training laboratories are now in place at each of the academic partner institutions. The Research Instrumentation Centers are in place and operated by a cadre of highly qualified staff scientists. Utilization of these facilities continues to grow with researches throughout the network. During Year 2 of the INBRE program, evaluation mechanisms were put in place to track the usage and growth of these shared facilities. Regular meetings were established among the directors of the Core Centers to share common operating strategies. Core Center fee waivers, partially funded by the INBRE grant, provide support for faculty and student researchers to access a center to establish baseline data sets for grant development or to launch student projects. The Delaware INBRE supported workshops for faculty, graduate students, and other researchers, to introduce them to available capabilities within the Network. The continuing focus for the Core Centers is on implementing best management practices to ensure efficient operation and on the long-term sustainability of these facilities. The Research Instrumentation Centers in the Delaware INBRE include: Instrumentation Center -- Core Center Staff - -2005 User Base [# of Faculty Groups] Bioimaging Kirk Czymmek, PhD, Elizabeth Adams, PhD, Deborah Powell, BS 48 Bioinformatics Douglas O¿Neal, PhD, Leo Genyuk, MS, Eric Garrison, MS, 17 DNA Sequencing & Genotyping Brewster Kingham, MS 42 Mass Spectrometry John Dykins, MS 6 Microarray Dianne York, MS 11 Nuclear Magnetic Resonance Steve Bai, PhD, Heather Egolf-Fox, BS 10 Plant Growth Chambers Robert Pekala, AME 10 Protein Production & Analysis Yu-Sung Wu, PhD, Angel Huang, MS 12 Accomplishments of the Instrumentation Core in Year 2 include: 1) A statewide research infrastructure of biomedical Core Center facilities with state-of-the-art instrumentation and professional staff is in place. 2) A new Core Center Web Portal (www.dbi.udel.edu/coreinstrumentation.html) is now available for faculty and students in the INBRE Network to locate research resources. The Core Center Web Portal describes available instrumentation, lists contact information for Core Center directors and staff, and provides pricing information for services. 3) Core Center Directors of several Core Centers at UD and DBI volunteered their time to help teach a key course in the Biotechnology Program at Delaware State University. In the 2005-2006 academic year, five seniors in Biotechnology needed an instrumentation course to fulfill their requirements for graduation in May 2006. Dianne York, Microarray Core Center Manager, acts as the course coordinator. She developed the syllabus and worked with the other core directors to arrange a schedule of lectures, and laboratory work, and coordinates the content and administering of the exams. This unique course is organized in a workshop format. 4) A Core Center Open House was held at DBI on November 11, 2005. The Open House event included presentations by all Core Center directors and was followed by tours and demonstrations in the labs. Over 30 attendees from across the Network attended the event. Based on the positive feedback, this event will now be held on an annual basis. 5) A Microarray Printing Workshop was conducted on January 18/19, 2006 by the Microarray Core Center. The workshop was fully book, and eight faculty and student researchers, form several partner institutions participated in the workshop. Based on the very positive evaluation, a follow-up workshop is in the planning stages. 6) In May 2005, DBI hosted an Introduction to Three Major Genome Browsers, presented by OpenHelix. The daylong workshop offered both an overview and hands-on session featuring the Ensembl Genome Browser, the UCSC Genome Browser, and the NCBI Map Viewer. 7) Several training workshops were conducted by the Bioimaging Core Center to introduce new imaging instrumentation, including a new Bioforce Microscope and a new Laser Tweezer option for the INBRE-funded Laser Capture Microdissection instrument. 8) The Bioinformatics Center is in the process of finalizing a software portal to ease access to the considerable computing resources for all members of the INBRE Network. This portal will allow users to submit new computing jobs into the computing server queue and also to improve access a broad set of life sciences and biomedical software programs available. 9) Core Center Directors meet on a bi-monthly basis to discuss best practices for common issues such as scheduling, billing, advertising, and joint workshops. 10) Professional education of scientific Core Center staff members continued through attendance at regional and national conferences and at internal workshops. 11) A Core Center Fee Waiver program is partially supported by the INBRE to provide free access for new researchers to the Core Centers for developmental projects that lead to the creation of baseline data for future grant proposals. 12) The partner institutions in the INBRE network added the following research instrumentation in Year 2: a) The University of Delaware added capability in the Bioimaging and Bioinformatics Centers, including: ¿ A Critical Point Dryer for FESEM sample preparation. This system provides the capability to prepare biological samples for imaging on the FESEM and maintain the cellular integrity of the sample through the drying process. This system has greatly expanded the user base on our FESEM. ¿ Additional memory modules to the Sun Biowolf Compute Cluster (96 GBs). This upgrade increases the performance of the cluster, with a much higher throughput of genomics searches. b) Delaware State University completed installation a major upgrade to the campus-wide IT structure, by securing Membership in the Internet 2 consortium as a Sponsored Participant. This capability, facilitated through a close collaboration between DSU and UD, will provide high-speed connectivity to departments and research facilities across the DSU campus. c) Delaware Technical & Community College purchased equipment to improve instruction in the Allied Health program and in the Sciences. This equipment includes: treadmills, ventilation analysis equipment, physics lab equipment, microscopes, balances, computers, chemistry and biology lab equipment d) Wesley College purchased instrumentation to expand undergraduate research experiences. This instrumentation will provide analysis techniques previously unavailable as well as training and presentation tools to provide this information to a broader audience within Wesley. This includes upgrades and software updates for the bioinformatics systems lab, laboratory setup for a new faculty member, and improved Internet connectivity to the campus.