COBRE on Membrane Protein Production and Characterization

COBRE 膜蛋白生产和表征

• 批准号: 8138958
• 负责人: ABRAHAM M LENHOFF
• 金额: $ 27.85万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-23 至 2012-09-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8138958
• 关键词: COBRE; Membrane; Protein; Production; Characterization

DESCRIPTION (provided by applicant): We propose to continue the COBRE on Membrane Protein Production and Characterization at the University of Delaware as a Phase III COBRE infrastructure center. The critical role of membrane proteins in biology is clearly reflected in the fact that they represent >30% of the human genome and are the targets of >50% of all therapeutics on the market today. Nevertheless, significant challenges remain in producing and characterizing them, especially within the realm of structural biology. Consequently the objectives of research in the COBRE will be to express, solubilize, purify and crystallize membrane proteins; to determine their structures; and to characterize their functions at the molecular level and in larger biological systems in both health and disease. These objectives are congruent with those of the Structural Biology component of the NIH Roadmap and the Protein Structure Initiative in addressing an area of critical importance for biomedical research. The infrastructure center will complement research in the laboratories of an interdisciplinary group of more than a dozen Pis, whose work is independently supported. To this end the center will include: research and instrumentation cores (Protein Expression and Biophysical Characterization; Structural Biology; and a group of Core Facilities co-funded with, among others, the Delaware INBRE); continuation of the current pilot research subproject program; a predoctoral training program modeled on the NIH T32 mechanism; and continuation of the existing annual symposium series. The administration of the center will be guided by an internal Steering Committee and will continue to benefit from the input of an External Advisory Committee, including for review of pilot project proposals. The research within the COBRE coupled with the capabilities provided by the infrastructure center will continue to give the University of Delaware distinctive strength in an area of research critical to biology and biomedicine. PUBLIC HEALTH RELEVANCE (provided by applicant): Membrane proteins exist in the membrane surrounding each cell and are critical to cellular growth, communication and nutrition, yet how these proteins function is still relatively poorly understood. The purpose of this center is to provide infrastructure for research to develop improved methods to produce and study membrane proteins, which can yield major benefits for basic biology and drug development.

描述（由申请人提供）：我们建议继续在特拉华州大学进行关于膜蛋白生产和表征的COBRE，作为第三阶段COBRE基础设施中心。膜蛋白在生物学中的关键作用清楚地反映在它们占人类基因组的>30%并且是当今市场上所有治疗剂的>50%的靶标的事实中。然而，在生产和表征它们方面仍然存在重大挑战，特别是在结构生物学领域。因此，COBRE的研究目标将是表达、溶解、纯化和结晶膜蛋白;确定其结构;并在分子水平和更大的健康和疾病生物系统中表征其功能。这些目标与NIH路线图的结构生物学部分和蛋白质结构倡议在解决生物医学研究的一个至关重要的领域是一致的。该基础设施中心将补充一个由十几名Pis组成的跨学科小组实验室的研究，该小组的工作得到独立支持。为此，该中心将包括：研究和仪器核心（蛋白质表达和生物物理表征;结构生物学;以及与特拉华州INBRE等共同资助的一组核心设施）;继续目前的试点研究子项目计划;以NIH T32机制为模型的博士前培训计划;以及继续现有的年度研讨会系列。该中心的行政管理将由一个内部指导委员会指导，并将继续受益于一个外部咨询委员会的投入，包括审查试点项目提案。COBRE内的研究加上基础设施中心提供的能力将继续为特拉华州大学在生物学和生物医学关键研究领域提供独特的优势。 公共卫生相关性（由申请人提供）：膜蛋白存在于每个细胞周围的膜中，对细胞生长、通讯和营养至关重要，但对这些蛋白的功能仍知之甚少。该中心的目的是为研究提供基础设施，以开发改进的方法来生产和研究膜蛋白，这可以为基础生物学和药物开发带来重大利益。