COBRE on Membrane Protein Production and Characterization

COBRE 膜蛋白生产和表征

基本信息

批准号：
8141171
负责人：
ABRAHAM M LENHOFF
金额：
$ 113.49万
依托单位：
UNIVERSITY OF DELAWARE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-10 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8141171
关键词：
COBRE Membrane Protein Production Characterization

项目摘要

DESCRIPTION (provided by applicant): We propose to continue the COBRE on Membrane Protein Production and Characterization at the University of Delaware as a Phase III COBRE infrastructure center. The critical role of membrane proteins in biology is clearly reflected in the fact that they represent >30% of the human genome and are the targets of >50% of all therapeutics on the market today. Nevertheless, significant challenges remain in producing and characterizing them, especially within the realm of structural biology. Consequently the objectives of research in the COBRE will be to express, solubilize, purify and crystallize membrane proteins; to determine their structures; and to characterize their functions at the molecular level and in larger biological systems in both health and disease. These objectives are congruent with those of the Structural Biology component of the NIH Roadmap and the Protein Structure Initiative in addressing an area of critical importance for biomedical research. The infrastructure center will complement research in the laboratories of an interdisciplinary group of more than a dozen Pis, whose work is independently supported. To this end the center will include: research and instrumentation cores (Protein Expression and Biophysical Characterization; Structural Biology; and a group of Core Facilities co-funded with, among others, the Delaware INBRE); continuation of the current pilot research subproject program; a predoctoral training program modeled on the NIH T32 mechanism; and continuation of the existing annual symposium series. The administration of the center will be guided by an internal Steering Committee and will continue to benefit from the input of an External Advisory Committee, including for review of pilot project proposals. The research within the COBRE coupled with the capabilities provided by the infrastructure center will continue to give the University of Delaware distinctive strength in an area of research critical to biology and biomedicine. PUBLIC HEALTH RELEVANCE (provided by applicant): Membrane proteins exist in the membrane surrounding each cell and are critical to cellular growth, communication and nutrition, yet how these proteins function is still relatively poorly understood. The purpose of this center is to provide infrastructure for research to develop improved methods to produce and study membrane proteins, which can yield major benefits for basic biology and drug development.

描述（由申请人提供）：我们建议继续在特拉华大学作为III期柯布雷基础设施中心的膜蛋白产生和表征的毛绒。膜蛋白在生物学中的关键作用显然反映在以下事实上，即它们占人类基因组的30％，并且是当今市场上所有治疗剂> 50％的靶标。然而，在产生和表征它们的范围内仍然存在重大挑战，尤其是在结构生物学领域。因此，在毛病中研究的目标将是表达，溶解，纯化和结晶膜蛋白。确定其结构；并在健康和疾病中在分子水平和较大的生物系统中表征其功能。这些目标与NIH路线图的结构生物学组成部分和蛋白质结构倡议相一致，以解决对生物医学研究至关重要的领域。基础设施中心将补充一个跨学科小组的实验室研究，由十几个PI，其工作得到独立支持。为此，该中心将包括：研究和仪器核心（蛋白质表达和生物物理表征；结构生物学；以及与特拉华州近亲共同资助的一组核心设施）；延续当前的试点研究副本计划；以NIH T32机制为模型的培训前培训计划；以及现有年度研讨会系列的延续。该中心的管理将由内部指导委员会指导，并将继续从外部咨询委员会的意见中受益，包括审查试点项目建议。毛cr中的研究以及基础设施中心提供的能力将继续在对生物学和生物医学至关重要的研究领域中为特拉华大学提供独特的力量。公共卫生相关性（由申请人提供）：每个细胞周围的膜中存在膜蛋白，对于细胞生长，通信和营养至关重要，但是这些蛋白质的功能仍然相对较差。该中心的目的是为研究提供基础设施，以开发改进的方法来生产和研究膜蛋白，这可以为基本生物学和药物开发带来重大好处。