LOUISIANA COBRE: OBESITY & DIABETES RES: GENOMICS CORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。彭宁顿生物医学研究中心基因组核心设施(PBRC-GCF)的目标是为路易斯安那州的LSI社区和周围机构提供高通量DNA测序、实时PCR和微阵列能力。方案预算资源中心--全球合作框架网站(http://gcf.pbrc.edii/).该系统于2001年春公布，已被该区域几个机构广泛用于自动提交样品、检索数据和开具帐单。该网站正在不断进行1：0的修改，以满足彭宁顿研究人员和周围科学界的需求。我们配备了一台AB13700(96毛细管)和两台ABI 3100(16毛细管)遗传分析仪(S)，有能力每天对超过10万个基对进行测序。PBRC-GCF微阵列设施生产来自小鼠(操作子，13,442和最近更新的16,463个寡核苷酸文库)和人类(Compugen.18,861个寡核苷酸文库)和几个由PBRC科学家创建的cDNA文库。幻灯片用配备了服务器臂的GeneMachine OmnigridMicroarrayer打印，并使用GS1 Lumonics ScanArray 5000进行扫描。生物信息学支持也将提供给研究人员，我们目前正在按照MIAMK概述的指导方针开发微阵列数据和信息的数据库，MIAMK是一个国际努力，旨在标准化微阵列数据，以提供关于微阵列实验的最少信息。PBRC-GCF配备了机器人(Biomek FX机器人工作站，Beckman-Coulter)来支持高通量DNA测序和微阵列技术，以及两个实时PCR序列检测系统(ABI 7700；ABI 7900)，用于验证微阵列结果和定量RT-PCR。PBRC-GCF由Leslie P.Kozak博士和Robert A.Koza博士指导，苏珊·纽曼在丽贝卡·麦凯布的协助下管理。除了提供服务外，核心还将开发新的方法，以加强PBRC科学家的研究活动。最初的开发将是一种CpG岛微阵列，用于研究与成人疾病相关的遗传变化的研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the Pennington Biomedical Research Centers Genomlcs Core Facility (PBRC-GCF) ).s to provide high-throughput DNA sequencing, real time PCR and microarray capabilities for the LSI) community and surrounding institutions in the State of Louisiana. The PBRC-GCF website (http://gcf.pbrc.edii/). made public during the spring of 2001, has been used extensively for automated sample submission, data retrieval and billing by several institutions in the region. The website is continually being modified 1:0 suit the needs of research staff at Pennington and the surrounding scientific community. We are equipped with one AB1 3700 (96-capillary) and two ABI 3100s (16-capillary) genetic analyzer(s) and have the capability to sequence more than Vz million basepairs a day. The PBRC-GCF micro array facility produces high-density micro arrays from mouse (Operon, 13,442 and recently updated 16,463 oligonucleotide libraries) and human (Compugen. 18,861 oligonucleotide library) and several cDNA libraries generated by scientists at PBRC. Slides are printed with a GeneMachine OmniGrid Microarrayer equipped with server arm and scanned using a GS1 Lumonics ScanArray 5000. Bioinformatics support will also be available to investigators and we are presently developing databases for microarray data and information following the guidelines outlined by MIAMK, an international effort to standardize microarray data to provide minimum information about a microarray experiment. The PBRC-GCF is equipped with robotics (Biomek FX robotic workstation, Beckman-Coulter) to support the high-throughput DNA sequencing and microarray technologies, and, two real-time PCR sequence detection systems (ABI 7700; ABI 7900) for verification of microarray results and quantitative RT-PCR. The PBRC-GCF is directed by Dr. Leslie P. Kozak and Dr. Robert A. KoZa arid managed by Susan Newman with assistance from Rebecca McCabe. In addition to services, the Core will develop new methodologies that will enhance the research activities of PBRC scientists. The initial development will be a CpG island microarray for the benefit of researchers working on cpigenetic changes in relation to adult disease.