MISSISSIPPI COBRE: CORE C: IN VITRO PHARMACOLOGY CORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。目的：用于开发精神药物治疗的天然产物的科学探索是一个研究不足的领域。体外药理学核心将提供几项与了解具有精神活性的天然产品的作用机制有关的功能，这些功能可以导致新疗法的开发。具体地说，核心C将提供在室内筛选化合物的生物活性并确定作用部位(S)以及作用机理(S)的能力。这些核心职能有赖于使用最先进的技术以及久经考验的实验室方法。使用多标签阅读器技术，核心将能够快速评估密西西比大学储存库中的化合物和提取物的特定受体活性。对铅化合物作用的机理研究将使用细胞电生理学和成像技术进行评估。受体亲和力测定和动力学分析将完善Core C的所有技术。表1概述了常规运行的分析方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objectives: The scientific exploration of natural products for the development of psychotropic therapies is an understudied area of research. The In Vitro Pharmacology Core will provide several functions related to understanding the mechanisms of actions of psychoactive natural products that can lead to the development of new therapies. Specifically, Core C will provide the ability in house to screen compounds for biological activity and determine the site(s) as well as mechanism(s) of action. These core functions are dependent on the use of state of the art technologies as well as time-tested laboratory methodologies. Using multi-label reader technologies, the core will be able to rapidly assess compounds and extracts held in the repositories at the University of Mississippi for specific receptor activities. Mechanistic studies into the actions of lead compounds will be assessed using cellular electrophysiology and imaging techniques. Receptor affinity determinations and kinetic analyses will round out Core C's repertoire of techniques. Table 1 presents an overview of the assays that are to be run routinely.