CORE B: CELL SEPARATION & ANALYSIS CORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。该核心的一个总体功能是提供基于物理、免疫、遗传和其他特征的细胞群分析和分离的最先进能力。这些能力在干细胞和祖细胞群体的工作中特别重要，而且在几乎所有其他类型的细胞生物学研究中也有应用。核心配备了几种仪器，以提供大多数细胞类型的分析，分离或消除。从Miltenyi MACS分选仪（自动磁性细胞分选仪）到四台Becton Dickinson流式细胞仪。最近，一个新的FACS Aria细胞分选系统已经获得。目前的参与者包括COBRE（COBRE血管生物学和COBRE干细胞生物学和再生医学）的研究人员。 此外，该核心正在开发中，以包括用于胚胎干细胞（ES）研究的资源。将提供干细胞研究的技术支持、培训和服务（特别是由Zack Wang提供）。小鼠和NIH批准的人ES细胞系将在适当的质量保证下保存。该设施提供的技术支持将包括细胞维护、转导和分化方面的实践指导。该设施还将为个体研究者提供ES细胞的短期培养和操作，并将为个体研究者提供ES细胞试剂（RNA、基因组DNA、蛋白质），而无需开发ES细胞工作的专门技术技能（或空间

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One overall function of this core is to provide state-of-the-art capabilities for the analysis and separation of cell populations based on physical, immunological, genetic, and other characteristics. These capabilities are of particular importance in working with stem and progenitor cell populations, but also find application in nearly all other types of cell biology research. The Core is equipped with several instruments in order to provide the analysis, isolation or depletion of most cell types. These range from a Miltenyi MACS Sorter (Automated Magnetic Cell Sorter) to four Becton Dickinson flow cytometers. Most recently, a new FACS Aria cell sorting system has been acquired. Current participants include investigators in both COBREs (COBRE Vascular Biology and COBRE Stem Cell Biology and Regenerative Medicine). In addition, this core is being developed to comprise a resource for the study of embryonic stem (ES) cells. Technical support, training, and services for stem cell research will be provided (esp by Zack Wang). Mouse and NIH-approved human ES cell lines will be maintained with appropriate quality assurance. Technical support provided by the facility will include hands-on instruction in the maintenance, transduction, and differentiation of the cells. The facility also will provide short-term culture and manipulation of ES cells for individual investigators, and will provide ready access to ES cell reagents (RNAs, genomic DNAs, proteins) for individual investigators without the need to develop specialized technical skills (or space) for ES cell work