COBRE RW HOSP: NEW STEM CELL BIOLOGY: CELL PHENOTYPING CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。本核心将集中于鉴定骨髓细胞移植后皮肤和骨骼肌细胞的性质。供体细胞和他们的特定谱系将确定和共聚焦和反褶积荧光显微镜共定位。供体细胞将在组织切片或细胞悬液中通过GFP、β -半乳糖苷酶的表达或男性DNA的存在进行追踪。鉴定供体细胞的谱系将通过标准形态学、地理位置和免疫组织化学来确定。将使用共聚焦或反卷积荧光显微镜确认供体和谱系标记的共定位。核心将为这些方法提供高水平的专业知识。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core will focus on identifying the nature of cells in skin and skeletal muscle after marrow cell transplantation. Donor cells and their specific lineages will be ascertained and colocalized by confocal and deconvolution fluorescent microscopy. Donor cells will be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of male DNA. The lineage of identified donor cells will be ascertained by standard morphology, geographical location and immunohistochemistry. Colocalization of donor and lineage markers will be confirmed using confocal or deconvolution fluorescent microscopy. The Core will provide high-level expertise for these approaches. .