Biofilm Growth on Functionalized Surfaces

描述(申请人提供)：生物膜中的细菌表现出独特的生理特征，与浮游培养的表型有很大不同。细菌生物膜最重要的特征之一是对抗菌剂和宿主免疫系统的攻击具有抵抗力。生活在生物膜中的细菌对抗生素的抗药性比浮游细菌高1000倍。与生物膜相关的医院感染和疾病目前是美国第四大死亡原因，仅次于心脏病、癌症和中风。无论使用什么抗生物膜方法，成功率都非常有限，但仍会在植入的设备上形成生物膜。在大多数情况下，与生物膜相关的感染只能通过高成本和不受欢迎的程序通过移除植入物来治愈。在这个项目中，我们建议从一个新的方向来对抗生物膜，直接处理附着的细菌和形成的生物膜。我们建议构建能够中断生物膜结构形成的功能表面。我们的假设是，在这些生物膜结构受损的功能表面形成的生物膜可能无法产生抗生素耐药性。这种生物膜中的细菌可能会保持对抗生素的敏感性，并可以通过普通的抗生素治疗来杀死。该项目的三个委员会目标是：1)构建具有所需结构和功能的表面；2)研究功能化表面上生物膜的形成动力学和形态；3)测试功能化表面上生物膜的抗生素敏感性，并研究不同抗生素处理方法的增敏机理，并用已建立的数学模型对数据进行拟合。公共卫生相关性：功能化表面上的生物膜生长叙述细菌可以附着在植入设备的表面并生长成菌群(称为生物膜)。与游离细菌不同，生物膜中的细菌很难通过普通的抗生素治疗而被杀死。生物膜相关感染和疾病目前是美国第四大死亡原因，仅次于心脏病、癌症和中风。这项研究的目标是开发新型表面，以防止与生物膜相关的感染和疾病。

DESCRIPTION (provided by applicant): Bacteria in biofilm show unique physiological characteristics that are much different from planktonic cultured phenotypes. One of the most important features of bacterial biofilms is their resistance to antimicrobial agents and the host immune system attacks. Bacteria living in biofilms can exhibit up to 1,000 time greater resistance to antibiotics than planktonic bacteria. Biofilm associated nosocomial (hospital acquired) infection and disease is currently the fourth leading cause of death in the United States, behind only heart disease, cancer and stroke. Regardless what anti-biofilm methods are used, the success rates are very limited and biofilms will nevertheless form on implanted devices. In most cases, biofilm related infections can only be cured by a high cost and undesirable procedure through the removal of the implants. In this project, we propose to fight against biofilm from a new direction by directly dealing with attached bacteria and formed biofilms. We propose to construct functional surfaces which can interrupt the formation of biofilm architecture. Our hypothesis is that biofilms formed on these functional surfaces with damaged biofilm architectures may not be able to develop antibiotic resistance. Bacteria in such biofilms may maintain antibiotic sensitivity and can be killed by ordinary antibiotic treatment. Three board objectives of this project are to 1) construct surfaces with desired structures and functions; 2) study biofilm formation dynamics and morphologies on functionalized surfaces; 3) test the antibiotic sensitivity of "biofilms" on functionalized surfaces and study sensitizing mechanisms using different antibiotic treatment approaches and fitting data with established mathematic models. PUBLIC HEALTH RELEVANCE: Biofilm Growth on Functionalized Surfaces Narrative Bacteria can attach to the surfaces of implant devices and grow into bacteria clusters (called biofilms). Unlike free bacteria, bacterial in biofilms can hardly be killed by ordinary antibiotic treatment. Biofilm associated infection and disease is currently the fourth leading cause of death in the United States, behind only heart disease, cancer, and stroke. The goal of this research is to develop new types of surfaces to prevent biofilm related infections and diseases.