Polymer Grafted Implant Surfaces with Biofilm Sensitizing Ability

具有生物膜敏化能力的聚合物接枝植入物表面

• 批准号: 7318286
• 负责人: JUN F LIANG
• 金额: $ 21.77万
• 依托单位: STEVENS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-15 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7318286
• 关键词: Accounting; Acids; Adopted; Affect; Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Antimicrobial Resistance; Architecture; Bacteria; Bacterial Adhesion; Bacterial Prostatitis; Catheters; Cause of Death; Cell membrane; Cell surface; Cells; Characteristics; Charge; Condition; Cystic Fibrosis; Dental caries; Devices; Diagnostic; Disabled Persons; Disease; Disruption; Drops; Educational process of instructing; Endocarditis; Excision; Exhibits; Figs - dietary; Goals; Grant; Growth; Heart Diseases; Heart Valves; Hour; Human; Immune system; Implant; In Vitro; Infection; Knowledge; Left; Life; Malignant Neoplasms; Medical; Methods; Microbial Biofilms; Modification; Molecular Conformation; Montana; Musculoskeletal; Necrotizing fasciitis; Nosocomial Infections; Operative Surgical Procedures; Osteomyelitis; Patients; Penile Prosthesis; Periodontal Diseases; Permeability; Phenotype; Physiological; Play; Polymers; Prevention; Procedures; Process; Prosthesis; Range; Rate; Research; Research Activity; Research Personnel; Role; Sepsis; Shunt Device; Solid; Starvation; Stroke; Surface; Time; United States; Universities; Venous; Ventricular; antimicrobial; antimicrobial drug; bacterial resistance; biliary tract; cell killing; cost; desire; experience; fight against; implantable device; implantation; killings; microbial; novel; nutrition; prevent; success; ventricular assist device

DESCRIPTION (provided by applicant): Bacteria in biofilm show unique physiological characteristics that are much different from planktonic cultured phenotypes. Biofilm associated nosocomial (hospital acquired) infection and disease is currently the fourth leading cause of death in the United States, behind only heart disease, cancer and stroke . Regardless what anti-biofilm methods are used, the success rates are still limited and biofilm will nevertheless form on implanted devices. In most cases, biofilm-induced infections can only be cured by a high cost and undesirable procedure through the removal of the implants. One of the most important features of bacterial biofilms is their resistance to antimicrobial agents and the host immune system attacks. Bacteria living in biofilms can exhibit up to 1000 time greater resistance to antibiotics than planktonic bacteria. We propose to fight against biofilm from a new direction by directly dealing with attached bacteria and formed biofilm to eradiate biofilm associated infection. We plan to construct novel polymer grafted surface which can sensitize attached bacteria or formed biofilm to host immune system attack and antibiotic treatment through disrupting biofilm architecture, increasing biofilm permeability, and blocking biofilm maturation. Three broad objectives are included in this AREA R15 project: Specific aim #1, create various polymer grafted surfaces with desired sensitivity to biofilm formation; Specific aim #2, study the formation dynamics and antibiotic sensitivity of biofilm on created polymer-grafted surfaces in vitro; Specific aim #3, study biofilm sensitizing mechanisms of polymer-grafted surfaces. This research holds significant intellectual merits for its first attempt of integrating the anti-biofilm activity directly into materials to develop biofilm sensitizing surfaces. Study of the biofilm sensitizing mechanism on pH-polymer grafted surfaces will enrich our knowledge and promote the research to win our battle with biofilms. In addition, this R15 grant is important for the investigator and his collaborators to continue their teaching and research activities at Stevens Tech. Bacteria can attach the surface of implant device and grow into bacteria clusters (called biofilm) in the patients' bodies. Unlike common bacteria, biofilm can hardly be killed by ordinary antibiotic treatment and thus implant device associated infection and disease is currently the fourth leading cause of death in the United States, behind only heart disease, cancer and stroke. The goal of this research is to develop new types of device surfaces to prevent biofilm formation and implantation association infections and diseases.

描述（由申请人提供）：生物膜中的细菌表现出独特的生理特征，与浮游培养的表型有很大不同。生物膜相关的医院感染和疾病目前是美国第四大死亡原因，仅次于心脏病、癌症和中风。无论使用何种抗生物膜方法，成功率仍然有限，生物膜仍会在植入装置上形成。在大多数情况下，生物膜引起的感染只能通过移除植入物的高成本和不受欢迎的手术来治愈。细菌生物膜最重要的特征之一是它们对抗菌剂和宿主免疫系统攻击的抗性。生活在生物膜中的细菌对抗生素的抵抗力比浮游细菌高1000倍。我们建议从一个新的方向来对抗生物膜，直接处理附着的细菌和形成的生物膜，以消除生物膜相关性感染。我们计划构建新型聚合物接枝表面，通过破坏生物膜结构，增加生物膜通透性，阻断生物膜成熟，使附着细菌或形成的生物膜对宿主免疫系统攻击和抗生素治疗敏感。AREA R15项目包括三个主要目标：具体目标#1，创造各种聚合物接枝表面，对生物膜形成具有所需的敏感性；具体目标#2，研究体外构建的聚合物接枝表面生物膜的形成动力学和抗生素敏感性；具体目标#3，研究聚合物接枝表面的生物膜增敏机制。本研究首次尝试将抗生物膜活性直接整合到材料中开发生物膜增敏表面，具有重要的知识价值。研究生物膜在ph -聚合物接枝表面的增敏机理将丰富我们的知识，促进我们与生物膜的斗争。此外，这项R15资助对于研究者和他的合作者在Stevens Tech继续他们的教学和研究活动很重要。