CORE--MOLECULAR BIOLOGY FACILITY

分子生物学技术已经彻底改变了生殖生物学家以快速、灵敏的方式解决特定生理问题的能力。然而，这种方法通常需要大量的时间投资来获得适当的专业知识和设备，额外的时间承诺来保持当前快速发展的领域，以及对最常规和重复的技术的质量控制的关键关注。许多分子实验的主要成本是提供可靠的试剂和探针所涉及的劳动力。一个集中的、装备良好的核心是提供重组DNA的最有效和最经济的方法

Molecular biology techniques have revolutionized the ability of reproductive biologists to address specific physiological questions in a rapid, sensitive manner. This approach, however, often requires a large time investment to acquire appropriate expertise and equipment, additional time commitments to keep current in a rapidly advancing field, and critical attention to quality control for even the most routine and repetitive techniques. A major cost for many molecular experiments is the labor involved to provide dependable reagents and probes. A centralized, well-equipped core is the most efficient and economical way to provide recombinant DNA methodology and reagents for projects to pursue innovative molecular work in reproductive medicine. The objective of the Molecular Core is to provide services, expertise, and equipment to research projects addressing clinical and basic questions in reproduction. Three research projects within the U54, and five additional projects approved by the Reproductive Sciences Branch require access to the Core for routine services of probe labeling (end-label, random priming, and single-strand RNA riboprobes), DNA isolation, plasmid growths and purification, DNA sequencing, and DNA fragment isolation and subcloning procedures. The core director and personnel will provide advice and expertise on the design of probes and expression vectors, various RNA and DNA quantitation methods, and specialized techniques such as library screening. Core personnel will provide timely, quality controlled service and reagents to approved projects on a priority basis. Approved investigators can access the core for specialized equipment to perform more advanced techniques on an availability basis. Finally, Core personnel will continue to improve and develop additional services and procedures as required by projects accessing the core, and update current services to provide the most sensitive, precise, and cost-effective reagents.