ESTABLISHMENT OF A SPF RHESUS MACAQUE COLONY

该项目的目标是提高国家生产无疱疹B病毒、SIV、SRV和STLV-1特异性病原体（SPF）的印度恒河猴（Macaca mulatta）的能力。 拟议的殖民地将产生高质量的遗传特征的动物用于艾滋病相关的研究由美国国立卫生研究院赠款设在西南地区灵长类动物研究中心（SRPRC）和其他研究机构在美国西南地区。 新SRPRC殖民地的大部分创始股票将通过美国空军向灵长类动物中心捐赠现有的SPF殖民地获得。 美国空军殖民地目前位于距离SRPRC 20分钟车程的优越设施中，可在本项目期间租赁给SRPRC。 总共215只动物（120只雌性育种动物、15只雄性育种动物和80只幼仔）将组成初始SPF群体。拟定的生产和收购时间表将使殖民地在4年内翻倍，达到430只动物的稳定状态。 这一目标群体规模将允许从04年开始每年收获70只动物，以满足艾滋病相关研究的关键需求。 此外，在前3年，每年将收获30至50只动物用于研究。将对殖民地产生的SPF猴进行血清学和遗传学表征。 将定期进行血清学检测和移除阳性动物，以确保SPF状态的维持。 将对SPF菌落的所有成员进行15个微卫星标志物的基因分型，这些微卫星标志物将用于评估母性和父性，并对殖民地进行遗传表征。 这些详细的遗传信息将被用于一个积极的遗传管理计划，旨在最大限度地减少近亲繁殖，最大限度地提高遗传变异性，并确保殖民地是完全纯种的。 将确定每只动物的Mamu-A O 1状态，并对部分殖民地采用选择性育种策略，以生产携带该MHC等位基因的动物。 将采用人口管理战略，通过制定最佳生产和收获时间表，确保殖民地的长期生存能力。SRPRC新设施的完工将使殖民地在5年赠款期结束时能够在中心居住。

The goal of this project is to increase the nation's capacity to produce Indian-origin rhesus monkeys (Macaca mulatta) that are specific pathogen-free (SPF) for herpes B virus, SIV, SRV, and STLV-1. The proposed colony will produce high quality genetically characterized animals for use in AIDS-related research by NIH grantees based at the Southwest Regional Primate Research Center (SRPRC) and at other research institutions in the southwestern region of the United States. Most of the founding stock of the new SRPRC colony will be obtained through donation of an existing SPF colony from the U.S. Air Force to the Primate Center. The U.S. Air Force colony is currently located a 20 minute drive from the SRPRC in excellent facilities that are available to be leased to the SRPRC for the duration of this project. A total of 215 animals (120 breeder females, 15 breeder males, and 80 juveniles) will comprise the initial SPF colony. The proposed production and acquisition schedule will result in doubling of the colony within 4 years to achieve a steady state of 430 animals. This target colony size will allow an annual harvesting of 70 animals per year beginning in Year 04 to fulfill critical needs in AIDS-related research. In addition, between 30 and 50 animals will be harvested for research during each of the first 3 years. The SPF monkeys produced by the colony will be well characterized serologically and genetically. Serological testing and removal of positive animals will be done on a routine basis to assure maintenance of SPF status. All members of the SPF colony will be genotyped for 15 microsatellite markers which will be used to assess maternity and paternity, and to genetically characterize the colony. This detailed genetic information will be used in an active genetic management program designed to minimize inbreeding, to maximize genetic variability, and to ensure that the colony is fully pedigreed. The Mamu-A O1 status of each animal will be determined, and a selective breeding strategy will be employed with a portion of the colony to produce animals which carry this MHC allele. Demographic management strategies will be used to ensure the long-term viability of the colony through development of optimal production and harvesting schedules. Completion of new facilities at the SRPRC will allow housing of the colony at the Center by the conclusion of the 5-year grant period.