Purchase of an ion trap mass spectrometer

描述（由申请人提供）：申请资金用于购买ThermoFinnigan LTQ Orbitrap质谱仪，该质谱仪将安装在Wistar研究所蛋白质组学核心设施中。该仪器将提供急需的提高鉴定蛋白质和翻译后修饰的能力，在复杂样品中具有高置信度。由8名调查人员组成的主要用户组将利用75%的仪器时间，8名次要用户将占仪器使用的额外21%。主要和次要用户群体中的大多数研究人员都有多个NIH资助的研究项目，这些项目将利用该仪器。因此，对主要用户群体完全可用的线性离子阱质谱仪是迫切需要的，并且将极大地推进NIH资助的Wistar研究所的研究。购买LTQ Orbitrap质谱仪比购买独立的LTQ仪器更可取，因为LTQ Orbitrap也将提供高分辨率和高精度的前体和碎片离子质量测量。这将极大地增强对复杂混合物中低丰度组分的鉴定，并提高置信度，同时也将提高对翻译后修饰鉴定的置信度。即使增加了所要求的LTQ Orbitrap，对2007年预期工作量的保守预测表明，该仪器和现有的LTQ都需要接近满负荷运行，每天24小时，每周7天，以满足需求。该仪器将被纳入蛋白质组学核心设备，该设备在过去6年中为许多学术研究人员提供了LC- MS/MS分析服务。2005年，总共有30个不同的实验室使用了这些服务，预计将来也会有类似数量的用户。Wistar研究所承诺为所要求的质谱仪提供住房、维护和人员配备，以确保质谱仪的使用寿命，并由研究所总裁兼首席执行官随附的信进行核实。

DESCRIPTION (provided by applicant): Funds are requested to purchase a ThermoFinnigan LTQ Orbitrap mass spectrometer that will be housed in the Wistar Institute Proteomics Core Facility. This instrument will provide critically needed increased capacity to identify proteins and posttranslational modifications with high confidence in complex samples. A major user group of 8 investigators will utilize 75% of instrument time and 8 minor users will account for an additional 21% of instrument use. Most investigators in both the major and minor user groups have multiple NIH funded research projects that will utilize this instrument. Hence, a linear ion trap mass spectrometer that is fully available to the major user group is critically needed and will greatly advance NIH funded research at the Wistar Institute. The purchase of an LTQ Orbitrap mass spectrometer is strongly preferred rather than a stand- alone LTQ instrument because the LTQ Orbitrap will also provide high resolution and high accuracy measurements of precursor and fragment ion masses. This will greatly enhance the identification of low abundance components in complex mixtures with improved confidence, and it should also improve the confidence of identifications of posttranslational modifications. Even with the addition of the requested LTQ Orbitrap, conservative projections of anticipated workload for 2007 indicate that both this instrument and an existing LTQ will need to be operated near full capacity, 24 hours per day, 7 days a week to keep up with demand. The requested instrument will be incorporated into a Proteomics Core Facility that has provided LC- MS/MS analysis services for the past 6 years to many academic investigators. In 2005, a total of 30 different laboratories used these services and similar numbers of users are anticipated for the future. The Wistar Institute is committed to housing, maintaining and staffing the requested mass spectrometer for its useful lifetime as verified by an accompanying letter from the Institute President and CEO.