FACSAria Flow Cytometer

描述（由申请人提供）：在过去的20年中，流式细胞术已经发展成为一种技术，用于检测细胞表面上的分子，监测从基因调控到蛋白质-蛋白质相互作用的细胞过程，以及快速分离显示所需表型的细胞（从细菌到哺乳动物）。直到最近三年，荧光激活的细胞分选仪能够快速检测和高速细胞分离，如我们的FACSVantage（1994年在NIH的支持下收购），是需要特殊实验室设施和高成本维护的大型复杂仪器。FACSAria是新一代台式细胞分选仪，在光学、检测灵敏度、射流、采集速率和分选能力方面都有显著进步。它是我们FACSVantage的及时和重要替代品。两个主要用户的到来，以及最近成立的医学寄生虫学系，对纽约大学的流式细胞术利用率提出了前所未有的要求。FACSAria将提供高速细胞分离、特殊的病原体遏制、额外的颜色检测、最大的操作者生产力和低维护成本。纽约州立大学没有类似的文书。在Doris Tse博士指导的CFAR流式细胞术核心的主持下，它将成为NYUSM，其附属机构和邻近研究机构的所有科学家的共享资源。CFAR执行委员会将与内部和外部咨询委员会一起监测使用情况。操作员工资、耗材和维护费将部分由NIH奖励（P30 AI 27742）支付，部分由正在进行的用户退款系统下的个人赠款支付。Tse博士拥有丰富的流式细胞术实践经验，将促进仪器利用和创新研究应用，验证数据解释，监督操作员并进行质量控制。该仪器将用于研究感染病原体的人类细胞和动物细胞。这些研究将促进我们对许多疾病的病因学和病理学的理解，包括艾滋病毒、结核病、哮喘、败血症、疟疾、肝炎、睡眠呼吸暂停和环境毒物暴露。这些信息将有助于制定或改进预防和治疗这些疾病的医疗程序。

DESCRIPTION (provided by applicant): Over the last 2 decades, flow cytometry has evolved into a technology that provides for the detection of molecules on the cell surface, the monitoring of cellular processes from gene regulation to protein-protein interaction, and the rapid isolation of cells, from bacterial to mammalian, that display desirable phenotypes. Until the last three years, fluorescence-activated cell sorters capable of multicolor detection and high-speed cell isolation, such as our FACSVantage (acquired in 1994 with NIH support), are large intricate instruments that require special laboratory facilities and high cost maintenance. The FACSAria is a new generation bench-top cell sorter with significant advances in optics, detection sensitivity, fluidics, acquisition rate, and sort capability. It is a timely and vital replacement for our FACSVantage. The arrival of two major users, and a recently established Department of Medical Parasitology, bring unprecedented demands on flow cytometry utilization at NYUSM. The FACSAria will provide high speed cell isolation, exceptional pathogen containment, additional color detection, maximal operator productivity, and low maintenance costs. There is no comparable instrument at NYUSM. Under the auspices of the CFAR Flow Cytometry Core directed by Dr. Doris Tse, it will be a shared resource available to all scientists at NYUSM, its affiliates, and neighboring research institutions. Usage will be monitored by the CFAR executive committee with an internal and external advisory board. Operator salary, consumable supplies, and maintenance fees will be covered in part by an NIH award (P30 AI27742), and charged in part to individual grants under an ongoing user-chargeback system. Dr. Tse, who has extensive hands-on flow cytometry experience, will promote instrument utilization and innovative research applications, validate data interpretation, supervise operators, and mandate quality controls. This instrument will be used to study human cells and animal cells infected with pathogens. These studies will advance our understanding of the etiology and pathology underlying many diseases, including HIV, TB, asthma, sepsis, malaria, hepatitis, sleep apnea, and environmental toxicant exposure. This information will potentiate the development or improvement of medical procedures towards their prevention and treatment.