STRUCTURE OF THE MITOCHONDRIAL RIBOSOME

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。最广泛研究的核糖体是细胞质的，通常来自细菌（特别是大肠杆菌）。coli）。这样的核糖体通常是约1/3的蛋白质和2/3的RNA质量。相反的是真正的线粒体核糖体，其具有相当小的RNA，通过添加许多细胞质核糖体中不存在的蛋白质来补充。线粒体核糖体通常只合成少数蛋白质，例如，牛线粒体基因组仅编码13种蛋白质，所有这些蛋白质都是负责氧化磷酸化的复合物的重要组成部分。因此，线粒体核糖体从结构和功能的角度来看都非常有趣。该项目旨在基于模型构建和冷冻电子显微镜技术开发这些核糖体的功能相关模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The most widely studied ribosomes are cytoplasmic, typically from bacteria (especially E. coli). Such ribosomes are typically about 1/3 protein and 2/3 RNA by mass. The reverse is true mitochondrial ribosomes, which have substantially smaller RNAs that are supplemented by the addition of many proteins not present in cytoplasmic ribosomes. Mitochondrial ribosomes typically synthesize only a handful of proteins ¿ for example, the bovine mitochondrial genome codes for only 13 proteins, all of which are essential components of the complexes responsible for oxidative phosphorylation. Mitochondrial ribosomes are thus extremely interesting from both structural and functional points of view. This project is aimed toward te development of functionally relevant models of these ribosomes based on model building and cryo-electron microscopy.