X-RAY STRUCTURES OF COMPONENTS INVOLVED IN MEMBRANE TRANSPORT

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。该项目旨在获得与蛋白质或其他分子跨膜运输有关的几个重要成分的高分辨率结构。一个主要的目标是结合到其伙伴核糖体或SecA的蛋白质转运通道的X射线结构。另一个目标是CCDA蛋白的X射线结构，它负责在细菌质膜上移动还原等价物。我们还试图获得高分辨率的网状结构，网状结构是参与膜小管形成的蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is designed to obtain high resolution structures of several important components involved in the transport of proteins or other molecules across membranes. A major goal are Xray structures of the protein-translocating channel bound to its partners, the ribosome or SecA. Another goal is an Xray structure of the CcdA protein, which is responsible for moving reducing equivalents across the bacterial plasma membrane. We are also trying to obtain a high resolution structure of the reticulons, proteins involved in the formation of membrane tubules.