STRUCTURE OF PROTEIN TRANSLOCATION COMPONENTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目旨在确定蛋白质转位组分的晶体结构。该项目的目标是深入了解蛋白质通过膜移动的机制。为此，我们试图获得通道本身的结构，与其伙伴结合的通道(ATPase SecA、核糖体或Sec62/63P复合体)的结构，以及具有易位多肽链的通道的结构。在过去，我们已经确定了来自古细菌的蛋白质易位通道SecY复合体的结构，以及与SecY通道结合的ATPase SecA的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims at determining crystal structures of protein translocation components. The goal of the project is to get insight into the mechanism by which proteins are moved through the membrane. To this end, we are trying to get structures of the channel on its own, of the channel bound to its partners (the ATPase SecA, the ribosome, or the Sec62/63p complex), and of the channel in action with a translocating polypeptide chain. In the past, we have determined the structures of the protein translocation channel, the SecY complex, from an archaebacterium, as well as the structures of the ATPase SecA bound to the SecY channel.