CORE--Tissue Culture and Immunoassay

这是一份组织培养和免疫测定核心（TCIC）设施的提案，其目的是为细胞培养维持、细胞和病毒纯化以及免疫测定性能提供集中资源，以支持德克萨斯大学医学分支（UTMB）题为”气道炎症信号传导”的计划项目中包含的五个项目。TCIC的具体目标是提供必要的基础设施：1。维持组织库， 人细胞系（主要是上皮来源的）; 2.制备蔗糖纯化的呼吸道合胞病毒池; 3.对人类和小鼠细胞因子和趋化因子进行免疫测定。对技术合作与信息中心设施的支持是合理的，因为该中心迫切需要一些生物试剂、方法学程序和免疫学测定，这些都是该方案项目所有五个项目的共同点。此外，集中采购试剂和进行测试将最大限度地降低整个中心的M&O和人员成本，并将实现检测和实验程序的最佳标准化。拟议的TCIC设施将利用UTMB目前可供计划项目调查人员使用的一些资源，包括儿童健康研究中心（CHRC）实验室，一个5,000平方米的实验室。英尺，TCIC实际所在的先进实验室设施。TCIC在UTMB校园内的中心位置将促进该计划项目的不同研究人员之间的沟通，从而更有效和及时地完成拟议的研究。

This is a proposal for a Tissue Culture and Immunoassay Core (TCIC) facility which purpose is to provide a centralized resource for cell culture maintenance, cellular and viral purification, and immunossay performance, in support of five projects included in the Program Project entitled" Signaling in Airway Inflammation" at University of Texas Medical Branch (UTMB). Specific objectives of the TCIC to provide infrastructure necessary to: 1. Maintain a tissue bank of human cell lines (mostly of epithelial origin); 2. Prepare pools of sucrose-purified respiratory syncytial virus; 3. Perform immunoassays for human and mouse cytokines and chemokines. The support of the TCIC facility is justified based on the critical need for a number of biological reagents, methodological procedures and immunological assays, common to all five projects of the Program Project. In addition, centralized acquisition of reagents and performance of the tests will minimize the cost of M&O and personnel for the whole center and will result in an optimal standardization of assays and experimental procedures. The proposed TCIC facility will take advantage of some resources currently available to the Program Project investigators at UTMB, including the Child Health Research Center (CHRC) laboratory, a 5,000 sq. ft., state-of-art laboratory facility where the TCIC will be physically located. The central location of the TCIC within the UTMB campus, will facilitate the communications among the different investigators of the Program project, resulting in a more efficient and timely completion of the proposed research.