PROJECT 3- TOXICOGENOMICS ARISTOLOCHIC ACID NEPHROPATHY

向1000多名居住在这些村庄的成年人 2005年春天。从每个受试者身上采集了血液和尿液样本，并进行了详细的问卷调查 管理。这些数据现已由训练有素的当地专业人员输入数据库 在缺失值和可疑极值时，广泛努力检查和/或核实信息 都被观察到了，回到村庄获取这些重要数据。因此，遗漏的值 保持在最低限度，将用于毒性基因组学研究的初始数据集预计将是 为2007年初的正式分析做好准备。 从1000名受试者身上获得的血清样本储存在斯拉沃恩斯基布罗德的公共卫生研究所， 克罗地亚，反映这些人肾功能的分析现已基本完成。白细胞DNA 是由Branko Brdar教授提取的，他是一名在Stony Brook接受培训的克罗地亚医学研究员，他的 合伙人。基于随机筛选，我们已经确定了提取的DNA的质量和数量 从这些样本中提取的SNP是非常好的，使我们能够继续进行拟议的SNP基因组分析。我们的 关于支持遗传病研究中心(CIDR)的建议，CIDR是SNP的集中设施 基因分型，已经得到NIEHS的批准。CIDR专家审查小组已经审查了这些信息 包含在目标1和目标2中，因为它们与基因分型分析有关。他们建议将CIDR工作人员分配到 CIDR董事会将考虑参与这一项目并支付大部分基因分型费用 州长们在10月份的会议上。 扩大我们与北京大学肾病学主任李晓梅教授的合作，我们 分析了27例确诊的AAN患者的尿路上皮细胞样本 PHS 398/2590(09/04版，2006年4月4日重新发布)第155页延续格式页

to more than 1,000 adults living in these villages during the spring of 2005. Blood and urine samples were collected from each subject and a detailed questionnaire was administered. These data have now been entered into a database by trained local professionals who have made extensive efforts to check and/or verify information when missing values and suspicious extreme values were observed, returning to the villages to obtain these important data. As a result, missing values have been kept to a minimum and the initial data set, which will be used in the toxicogenomics study, is expected to be ready for formal analysis early in 2007. Serum samples obtained from 1,000 subjects are stored at the Institute of Public Health in Slavonski Brod, Croatia, and the analyses reflecting renal function of these persons are now largely complete. Leukocyte DNAs were extracted by Professor Branko Brdar, a Stony Brook-trained Croatian medical researcher, and his associates. Based on random screens, we have determined that the quality and quantity of DNA extracted from those samples is excellent, permitting us to proceed with the proposed SNP genomic analyses. Our proposal for support to the Center for Inherited Disease Research (CIDR), a centralized facility for SNP genotyping, has been approved by NIEHS. The CIDR expert review panel has reviewed the information contained in Aims 1 & 2 as they relate to genotyping analyses. Their recommendation to assign CIDR staff to participate in this project and to cover most of the costs of genotyping will be considered by the CIDR Board of Governors at their October meeting. Extending our ongoing collaboration with Prof. Xiaomei Li, Head of Nephrology at the University of Beijing, we analyzed 27 samples of exfoliated urothelial cells from patients with documented AAN who are being followed PHS 398/2590 (Rev. 09/04, Reissued 4/2006) Page 155 Continuation Format Page