Electron Microscopy Core

本计划项目的EM核心有两个相关的目的--支持 在这笔赠款的四个项目中提出的研究，并促进正在开发的方法的应用 在这些项目中需要合作和独立的努力。核心的主要成分是电子。 哈佛医学院(HMS)和布兰迪斯大学(Brandeis University)的显微镜设施。HMS设施将 有四台FBI显微镜：一台Tecnai F20，一台Tecnai T12，一台CM10和一台Polara F30。F30、F20和 T12显微镜配备了低温工作台；F30和F20配备了场发射枪。布兰代斯夫妇 该设施还配备了四台FBI仪器：Morgagni M268、EM20、CM12和Tecnai F30。最后 其中三个有低温阶段；F30有一个场发射喷枪和一个能量过滤器。核心支持以下操作 这些显微镜和每个地点设施经理的工资，以及必要的辅助 设备。核心也有一个计算组件。它维护和升级所有标准的EM软件， 包括FREALIGN、IMagic和Spider等程序套件，通过结构生物学 HMS分子和细胞动力学中心建立的计算网格(SBGrid)。最后， 通过提供EM和计算设施，核心允许这些项目的P.I.S与 不同机构和机构的其他小组将对博士后研究员和学生进行相关方法方面的培训。 相关性：电子显微镜是连接低分辨率和高分辨率的关键实验工具 结构方法。大型组件将成为未来人类疾病干预的目标，以及电子 显微镜将是联系这些复合体的结构和功能的中心

The EM core of this Program Project has two related purposes - to support instrumentation essential for the research proposed in the four projects of this grant and to facilitate application of the methods being developed in these projects to collaborative and independent efforts. The principal components of the core are the electron microscopy facilities at Harvard Medical School (HMS) and at Brandeis University. The HMS facility will house four FBI microscopes: a Tecnai F20, a Tecnai T12, a CM10, and a Polara F30. The F30, the F20, and the T12 microscopes are equipped with cryo-stages; the F30 and the F20 have field-emission guns. The Brandeis facility also houses four FBI instruments: a Morgagni M268, and EM20, a CM12, and a Tecnai F30. The last three have cryo-stages; the F30 has a field-emission gun and an energy filter. The core supports the operation of these microscopes and the salary of a facilities manager at each location, as well as necessary ancillary equipment. The core also has a computational component. It maintainsand upgrades all standard EM software, including program suites such as FREALIGN, IMAGIC, and SPIDER, through a structural biology computational grid (SBGrid) established by the Center for Molecular and Cellular Dynamics at HMS. Finally, by providing EM and computational facilities, the core allows the P.I.'s of these projects to collaborate with other groups at various institutionsand to train postdoctoral fellows and students in the relevant methods. Relevance: Electron microscopy is the key experimental tool that bridges between low- and high-resolution structural methods. Large assemblies will be targets of future interventions in human disease, and electron microscopy will be central for relating structure and function of these complexes