Core--Viral, Cellular and Molecular Biology Facility

病毒/细胞/分子核心的目的是为病毒学， 分子和原代细胞为基础的研究，以提高生产力和效率的既定艾滋病毒研究人员在宾夕法尼亚大学/Wistar/CHOP社区，促进新的研究人员进入艾滋病研究领域，并促进这些研究人员之间的科学合作。核心规定：（1）生物安全3级设施，供研究人员在无法进入单独的隔离设施或需要比目前更高的隔离水平时进行隔离实验;（2）代表不同发病机制的原型、突变体和特征明确的主要HIV-1分离物的中央储存库;（3）用于初级HIV-1分离、滴定和表征的服务;（4）额外的病毒学服务，包括病毒储备的生长、滴定和QA，以及具有成本效益的HIV p24和SIV p27 ELISA测定;（5）通过白细胞去除术的特定基因型（即，CCR 5 δ 32纯合）的高容量初级PBMC， 和;（6）支持使用定量实时PCR技术，包括机器使用和成本效益优化的引物/探针组以及为核心用户批量制备的试剂。该中心还提供以下方面的培训方案：（a）为新从事艾滋病毒研究的实验室人员提供艾滋病毒生物安全做法，或为现有研究人员提供进修方案;（B）提供使用实时定量PCR技术的培训，包括定制引物开发和数据分析软件;（c）向新的研究人员提供病毒学技术的实用方面的援助。我们期望，该核心将继续提高现有艾滋病毒调查人员的生产力和合作，并促进非艾滋病毒调查人员进入该领域，同时提供一种机制，加强实验室生物安全。

The purpose of the Viral/Cell/Molecular Core is to provide comprehensive support for virological, molecular and primary cell-based studies, in order enhance the productivity and efficiency of established HIV investigators within the Penn/Wistar/CHOP community, facilitate the entrance of new investigators into the field of AIDS research, and promote scientific collaborations among these investigators. The Core provides: (1) a carefully supervised facility for Biosafety Level 3 facility for the performance of containment experiments by investigators without access to individual containment facilities or who require a level of containment greater than currently available; (2) a central repository of prototype, mutant, and well-characterized primary HIV-1 isolates representing different aspects of pathogenesis; (3) a service for primary HIV-1 isolation, titration and characterization; (4) additional virologic services including growth, titration and QA of viral stocks, and cost-efficient HIV p24 & SIV p27 ELISA assay; (5) high volume primary PBMC of specified genotype (ie, CCR5 delta32 homozygous) by leukapharesis, and; (6) support for the use of quantitative real-time PCR technology including machine use and cost-efficient optimized primer/probe sets and reagents prepared in bulk for Core users. The Core also provides training programs in (a) HIV biosafety practices for lab personnel new to HIV research or refresher programs for current research personnel; (b) training in use of realtime quantitative PCR technology including custom primer development & data analysis software, and; (c) assistance to new investigators with practical aspects of virological techniques. It is our expectation that this Core will continue to enhance productivity and collaborations among existing HIV investigators and facilitate the entrance of non-HIV investigators into the field, while providing a mechanism to enhance laboratory biosafety.