Core--Viral, Cellular and Molecular Biology Facility

病毒/细胞/分子核心的目的是为病毒学、 分子和初级细胞研究，以提高宾夕法尼亚大学/维斯塔尔大学/CHOP社区内现有艾滋病毒调查人员的生产力和效率，促进新的调查人员进入艾滋病研究领域，并促进这些调查人员之间的科学合作。该中心提供：(1)生物安全3级设施的严密监督设施，供调查人员在不接触个别封闭设施或需要高于目前可用的封闭水平的情况下进行封闭实验；(2)代表致病机制不同方面的原型、突变和特征良好的初级艾滋病毒-1分离株的中央资料库；(3)初级艾滋病毒-1分离、滴定和表征服务；(4)额外的病毒学服务，包括病毒库存的生长、滴定和质量保证，以及具有成本效益的艾滋病毒p24和SIV p27酶联免疫吸附试验；(5)通过白血球排出大量特定基因(即CCR5突变32纯合子)的原代PBMC， 以及；(6)支持使用定量实时聚合酶链式反应技术，包括机器使用和为核心用户批量准备的具有成本效益的优化的引物/探针组和试剂。该中心还提供以下方面的培训：(A)为新接触艾滋病毒研究的实验室人员提供艾滋病毒生物安全实践，或为现有研究人员提供进修计划；(B)使用实时定量聚合酶链式反应技术(包括定制引物开发和数据分析软件)的培训；以及(C)向新的研究人员提供病毒学技术实用方面的协助。我们期望，这一核心将继续提高现有艾滋病毒调查人员之间的生产力和合作，并便利非艾滋病毒调查人员进入该领域，同时提供一个加强实验室生物安全的机制。

The purpose of the Viral/Cell/Molecular Core is to provide comprehensive support for virological, molecular and primary cell-based studies, in order enhance the productivity and efficiency of established HIV investigators within the Penn/Wistar/CHOP community, facilitate the entrance of new investigators into the field of AIDS research, and promote scientific collaborations among these investigators. The Core provides: (1) a carefully supervised facility for Biosafety Level 3 facility for the performance of containment experiments by investigators without access to individual containment facilities or who require a level of containment greater than currently available; (2) a central repository of prototype, mutant, and well-characterized primary HIV-1 isolates representing different aspects of pathogenesis; (3) a service for primary HIV-1 isolation, titration and characterization; (4) additional virologic services including growth, titration and QA of viral stocks, and cost-efficient HIV p24 & SIV p27 ELISA assay; (5) high volume primary PBMC of specified genotype (ie, CCR5 delta32 homozygous) by leukapharesis, and; (6) support for the use of quantitative real-time PCR technology including machine use and cost-efficient optimized primer/probe sets and reagents prepared in bulk for Core users. The Core also provides training programs in (a) HIV biosafety practices for lab personnel new to HIV research or refresher programs for current research personnel; (b) training in use of realtime quantitative PCR technology including custom primer development & data analysis software, and; (c) assistance to new investigators with practical aspects of virological techniques. It is our expectation that this Core will continue to enhance productivity and collaborations among existing HIV investigators and facilitate the entrance of non-HIV investigators into the field, while providing a mechanism to enhance laboratory biosafety.