LSI Doctoral Training Centres - Doctoral Training Centre in Cell & Proteomic Technologies

列出拟议中心的主要专题范畴及需要。主题是蛋白质组学和相关的细胞技术。这将包括：小样本蛋白质组学：包括用于临床应用的组织和生物流体样本，稀有蛋白质分析，致力于单细胞蛋白质组学。蛋白质富集与分离：亲和富集、新的色谱方法和蛋白质复合物的快速分离与分析。蛋白质、核酸和药物阵列：蛋白质捕获阵列和新的读取阵列方法，包括质谱、荧光寿命、荧光相关和量子点技术。定量蛋白质组学：特别是翻译后修饰。芯片实验室，特别是微流体和单细胞操作。生物光子学和成像：这些技术在单细胞研究和组织分析中发挥着越来越重要的作用。质谱法：应用高场FTICR， MS成像和有限样品导入的新方法。组织和细胞工程：特别是细胞与表面的相互作用，生物力学线索的感知和细胞组合之间的相互作用。生物信息学和计算科学：特别是系统生物学的数据生成和分析。随着基因组学的革命，现在迫切需要训练有素的新研究人员在生物学、物理科学和工程学之间的跨学科界面工作，以解决分子和单细胞水平上的关键生物力学问题；这个中心将提供这些研究人员。

List the broad thematic areas and need for the proposed centre.The main theme is proteomic and related cell technologies. This will include:Small sample proteomics: including tissue and biofluid samples for clinical applications, analysis of rare proteins, working towards single cell proteomics.Protein enrichment and separation: affinity enrichments, new chromatography methods and rapid isolation and analysis of protein complexes.Protein, nucleic acid and drug based arrays: protein capture arrays and new methods of reading arrays including mass spectrometry, fluorescence lifetime, fluorescence correlation and quantum dot technologies.Quantitative proteomics: especially for post-translational modifications.Lab-on-a-chip, especially microfluids and single cell manipulation.Biophotonics and imaging: these technologies are playing an increasingly important role in single cell studies and tissue analysis.Mass spectrometry: new methods applying high-field FTICR, MS imaging and sample introduction for limited samples.Tissue and cell engineering: especially the interaction of cells with surfaces, sensing of biomechanical cues and interactions between assemblies of cells.Bioinformatics and computational science: especially data generation and analysis for systems biology.Following the genomics revolution there is now a critical need for new researcher trained to work at the interdiscplinary interfaces between biology, physical sciences and engineering to tackle key biomechanical problems at the molecular and single cell level; this centre will provide these researchers.