Microwave-Assisted Electrospray Ionization for LC/MS

描述（由申请人提供）：该小型企业创新研究第一阶段项目将测试一种新方法的可行性，该方法通过微波诱导热化实现电喷雾电离（ESI）质谱的增强汽化。将采取两种方法来诱导加热来自毛细管的流出物。第一种策略是将微波腔（Evenson型腔）直接放置在柱的尖端，产生必要的功率（约1000 W）。8 W）以增强所得液滴流的蒸发。该技术的优势在于，微波驱动器之前已经过测试并证明可以提供足够的能量。第二个要测试的微波策略是将毛细管尖端刚好放置在RF驱动线圈上方（也是ca. 8 W）。已经发现这种RF线圈设计是可行的，能够提供可调谐的RF能量，该RF能量也可以被脉冲化以更好地控制加热过程。在通过微波场之前和之后，将进行颗粒直径的真实的尺寸测量，从而更好地理解微波辐射对液滴干燥过程的影响。 公共卫生相关性：使用ESI喷雾器作为源的LC/MS领域非常广泛，为药理学、医学、免疫学、石油和蛋白质组学等行业的不同领域提供分析支持。特别是在药物工业中，已经尝试增强LC/MS分析，以扩展ESI的传统极性检测能力，以包括低极性至非极性化合物。这导致了最近将光电离（PI）源与ESI结合，也称为ESPI。对于这种新出现的多模式源的一个挑战是对于有效PI的高效干燥过程的要求;然而，当前ESI喷雾器技术在所得流可以被加热的程度上是有限的。这使得我们的团队研究微波辅助技术克服这一挑战的可能性。拟议的活动将对整个社会产生广泛而深远的影响，提供有价值的信息，可以导致使用ESI LC/MS改进化合物的分析检测。实际结果是改善基础学术研究和全行业对社会重要化合物的检测。这项工作还涉及学术合作，并将促进研究生的教学，培训和学习。最后，该项目具有爆炸性商业增长的潜力，这将刺激经济发展。

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project will test the feasibility of a new method for achieving enhanced vaporization via microwave-induced thermalization for electrospray ionization (ESI) mass spectrometry. Two approaches will be taken to induce heating of the effluent from the capillary. The first strategy is the placement of a microwave cavity (Evenson-type cavity) directly to the tip of the column, generating the necessary power (ca. 8 W) to enhance vaporization of the resulting droplet stream. The advantage of this technology is that the microwave driver has been previously tested and demonstrated to provide sufficient energy. A second microwave strategy to be tested is the placement of the capillary tip just above an RF-driven coil (also ca. 8 W). This RF-coil design has been found to be feasible, capable of supplying tuneable RF-energy that can also be pulsed to better control the heating process. Real- time sizing of particle diameter both before and after passage through the microwave field will be taken, allowing for a better understanding of the affects of microwave radiation on the drying process of the droplet. PUBLIC HEALTH RELEVANCE: The field of LC/MS using the ESI sprayer as a source is extensive, providing analytical support for diverse fields within industries such as pharmacology, medical, immunological, petrol and proteomics. Especially within the pharmacological industry, there have been attempts to enhance LC/MS analysis to extend the traditionally polar-only detection capabilities of ESI to include low- to non-polar compounds. This has resulted in the recent incorporation of photoionization (PI) sources with ESI, also termed ESPI. One challenge to this newly emerging multimode source is the requirement of a highly efficient drying process for effective PI; however, the current ESI sprayer technology is limited in the extent to which the resulting stream can be heated. This has led our group to investigate the possibility of microwave-assisted techniques to overcome this challenge. The proposed activity will have a broad and profound impact on society as a whole by providing valuable information that can lead to improved analytical detection of compounds using ESI LC/MS. The practical outcome is to improve both fundamental academic research and industry-wide detection of socially significant compounds. This work also involves an academic collaboration and will promote teaching, training, and learning of graduate students. Finally this project has the potential for explosive commercial growth, which will stimulate economic development.