BIOINFORMATICS/MICROARRAY SATELLITE CORE-OSU

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 俄克拉荷马州立大学(OSU)微阵列核心设施(OMCF)及其相关的生物信息学小组BioInfOSU提供设施、咨询、教育和培训。他们的设施包括现代仪器，用于制备寡核苷酸和多核苷酸微阵列，用于将其与标记的目标核酸杂交，用于扫描杂交后斑点的荧光强度，以及用于计算分析结果。员工包括一名具有丰富生物信息学经验的博士生教员级别的微阵列专家、一名精通微阵列数据处理的应用程序员和一名系统管理员。所有人员职位都已过渡到长期全职职位，以确保继续支持俄克拉荷马州立大学及以后的研究。为了证明这些工作人员和设施能够刺激和促进功能基因组学研究，OMCF和BioInfOSU为所有级别的研究人员提供讲习班和咨询服务，并普遍提供他们的资源。由超过25名首席调查人员领导的小组利用OSU微阵列和生物信息学设施研究了各种生物：美洲Amblyomma americanum(孤星扁虱)、空肠弯曲杆菌、狗牙根(百慕大草)、多刺埃默氏菌、多毛棉(棉花)、流感病毒、番茄(番茄)、褐家鼠(老鼠)、高粱、聚合孢子虫。PCC6803、普通小麦、紫花苜蓿、小鼠、智人、杜氏杆菌、尼杜氏曲霉、中国仓鼠卵巢细胞、牛和各种病原微生物。该机制支持的工作导致在同行评议的期刊上发表文章(见G节)。除了培养研究生和博士后外，领导这些实验室的科学家每年都会欢迎无数本科生进入他们的实验室参与研究。来自客户的反馈不断提高设施提供服务和建议的能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Oklahoma State University (OSU) Microarray Core Facility (OMCF) and its associated bioinformatics group, BioinfOSU, provide facilities, consultation, education and training. Their facilities include modern instruments for the preparation of microarrays of oligo- and polynucleotides, for their hybridization to labeled target nucleic acids, for scanning the fluorescent intensities of spots after hybridization and for computational analysis of the results. Staff includes a Ph. D. faculty-level microarray specialist with extensive bioinformatics experience, an applications programmer versed in microarray data handling and a systems administrator. All personnel positions have been transitioned to permanent full-time positions insuring the continued support of research at Oklahoma State University and beyond. To demonstrate that such staff and facilities stimulate and facilitate research in functional genomics, OMCF and BioinfOSU provide workshops and consultation services to investigators of all levels, as well as making their resources generally available. Groups led by more than 25 Principal Investigators use OSU microarray and bioinformatics facilities to study a diverse range of organisms: Amblyomma americanum (lone star tick), Campylobacter jejuni, Cynodon dactylon (bermuda grass), Emericella nidulans, Gossypium hirsutum (cotton), Influenza viruses, Lycopersicon esculentum (tomato), Rattus norvegicus (rat), Sorghum, Synechocystis sp. PCC6803, Triticum aestivum (bread wheat), Medicago truncatula, Mus musculus, Homo sapiens, Dunaliella spp., Aspergillis nidulans, Cricetus (Chinese hamster ovary cells), Bos taurus (cow), and various pathogenic microbes. The work supported by the facility has led to publications in peer-reviewed journals (see section G). In addition to training graduate and postdoctoral students, the scientists leading these laboratories annually welcome numerous undergraduate students into their laboratories to participate in the research. Feedback from clients continually improves the ability of the facilities to provide services and advice.