DESIGN OF COLORIMETRIC AND CHIROPTICAL SENSORS FOR ABUSED NARCOTICS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在首席研究员Andrea Holmes的指导下，化学助理教授Doane的本科生 学院将参与一个正在进行的研究项目，以开发新的高度选择性和敏感的分子传感器，在非法毒品的存在下改变颜色。一个组合的方法将被用来找到这些传感器，通常 滥用药物，如可卡因，约会强奸药物，如lunitrazepam和GHB，和甲基苯丙胺。该项目涉及使用现代分析技术，例如通过紫外/维斯和荧光光谱法测定结合常数， 使用基于阿托西肽的结合剂（适体）和组合96孔板方法，从而使本科化学研究与当前使用的主流技术相结合。五年期研究和教育的预期里程碑是：确定更好的染料，与使用可卡因的SELEX已经获得的适体形成强复合物;通过使用固定的氟硝西泮和SELEX找到更特异的适体并测试染料;将方法扩大到其他滥用的麻醉品，如伽马-羟丁酸和甲基苯丙胺;以及将传感器添加到实地监测的坚实支持。 由执法部门和卫生专业人员进行检测。所有的工作都将是完全可见的，并与教育相结合。 Doane化学计划的目标。 智力优势：该项目依赖于物理，有机，分析和生物学等跨学科领域的协同作用。 法医化学教师/本科生研究团队将利用创造性和原创性的组合技术， 导致使用适体和固相技术的简单的“混合和测量”传感测定， 已扩大到其他滥用麻醉品，因此与人类健康高度相关。丰富的学习环境， 研究，更好的教学材料，协作学习，跨学科研究，并结合现代 工具被认为是学生赋权和有效学习科学的一些最重要的关键 教育 更广泛的影响：如果成功，该研究将导致开发新的，强大的，敏感的和选择性的分子。 传感器用作手持比色现场测试，可用于执法，卫生专业人员，分析 实验室，法医科学家和毒品袭击或过量的潜在受害者。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Under direction of principal investigator Andrea Holmes, assistant professor of chemistry, undergraduate students at Doane College will participate in an ongoing research project to develop new highly selective and sensitive molecular sensors that change color in the presence of illegal narcotics. A combinatorial approach will be used to find these sensors for commonly abused drugs such as cocaine, date-rape drugs like lunitrazepam and GHB, and methamphetamine. The project involves the use of modern analytical techniques, such as the determination of binding constants by UV/Vis and fluorescence spectroscopy, work with oligonucleotide-based binders (aptamers), and combinatorial 96-well plate methods, thus allowing the integration of undergraduate chemistry research with currently used mainstream technology. Anticipated milestones for the five-year period of research and education are to determine better dyes that form strong complexes with aptamers already obtained by SELEX using cocaine, find more specific aptamers by using immobilized flunitrazepam and SELEX and test the dyes, expand the methodology to other abused narcotics such as GHB and methamphetamine, and add the sensor to solid support for field testing by law enforcement and health professionals. All the work will be fully visible and integrated with the educational goals of the Doane chemistry program. Intellectual Merit: The project relies on the synergism of the interdisciplinary fields of physical, organic, analytical, and forensic chemistry. The faculty/undergraduate research team will utilize creative and original combinatorial techniques that lead to simple "mix and measure" sensing assays using aptamer and solid-phase technology that have the potential to be expanded to other abused narcotics and is therefore highly relevant to human health. Learning environments that are rich in research, better instructional materials, collaborative learning, interdisciplinary research, and incorporation of modern instruments are recognized as some of the most important keys to student empowerment and effective learning in science education. Broader Impact: If successful, the research will lead to the development of novel, robust, sensitive, and selective molecular sensors for use as hand-held colorimetric field-tests that could be used by law enforcement, health professionals, analytical laboratories, forensic scientists, and potential victims of drug assault or overdosing.