METABOLISM STUDIES OF E COLI

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该生物分析项目的具体目标是：1)开发能够分解复杂碳水化合物混合物的毛细管电泳法；2)应用这些方法来分析具有生物医学重要性的胃肠道生态系统中的营养可获得性。更具体地说，将检查小鼠肠道粘液的体内营养含量，这是感染大肠杆菌的栖息地。这个项目将是俄克拉荷马大学的泰瑞尔·康韦博士和东南部俄克拉荷马州立大学的蒂姆·史密斯博士共同努力的成果。小鼠定植研究中的突变分析显示，几种糖似乎对大肠杆菌的定植很重要。这些发现的一个重要扩展是使用化学方法来证明这些糖在粘液中可用，并确定大肠杆菌在体内对个别粘液衍生糖的偏好顺序。在这项研究中，毛细管电泳法利用体外和体内模型监测了不同菌株的碳水化合物和氨基酸的代谢。其目标是确定肠道细菌的实际营养可获得性。这一合作提供了对胃肠道病原体如何获得感染宿主并开始疾病过程所需的营养的更深层次的理解，并应建立在这一模式系统的知识基础上。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim of this bioanalytical project is to: 1) develop capillary electrophoresis-based assays capable of resolving complex mixtures of carbohydrates and 2) apply the methodologies to analyze nutrient availability in a gastrointestinal ecosystem of biomedical importance. More specifically, the in vivo nutrient content of mouse intestinal mucus, which is the habitat for infecting E. coli, will be examined. This project will be a collaborative effort between Dr. Tyrrell Conway of the University of Oklahoma and Dr. Tim Smith at Southeastern Oklahoma State University. Mutational analysis in mouse colonization studies revealed several sugars that appeared to be important for E. coli to colonize. An important extension of these findings is to use chemical methods to prove these sugars are available in mucus and to determine E. coli's in vivo order of preference for the individual mucus-derived sugars. In this study, capillary electrophoresis was utilized to monitor the carbohydrate and amino acid metabolism of various E. coli strains using both in vitro and in vivo models. The goal is to determine the actual nutrient availability to enteric bacteria in the intestine. This collaboration is providing a deeper understanding of how gastrointestinal pathogens acquire the nutrients necessary to infect their host and begin the disease process and should build on the knowledge of this model system.