IRON-BINDING COMPOUNDS PRODUCED BY OPEN-WATER MARINE FUNGI

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 许多微生物产生低分子量的铁（III）特异性结合化合物，称为铁载体，以在铁限制期间竞争铁。 从陆地细菌和真菌以及海洋细菌中分离的铁载体显示出作为生物医学试剂和控制微生物生长的前景。 迄今为止，没有铁结合化合物产生的海洋真菌已结构特征。 海洋真菌产生的铁载体提供了一个尚未开发的新的生物活性化合物的来源。 拟议的项目将提供海洋真菌铁载体结构的第一个例子。 该项目的具体目标是： 1. 筛选在铁限制条件下生产铁结合化合物的远洋真菌菌株。 2. 从海洋真菌菌株中分离铁结合化合物。 3. 使用氨基酸分析、核磁共振和质谱法确定海洋真菌产生的铁载体的结构。 本科生参与研究是他们的培训和教育的重要组成部分。 这项研究将与东北州立大学的本科生合作完成。 学生将有机会学习微生物学技术，天然产物分离和纯化技术，包括固相萃取和反相HPLC，以及结构表征方法，包括氨基酸分析，脂肪酸分析，质谱法，以及1D和2D NMR。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many microorganisms produce low-molecular-weight, iron(III)-specific binding compounds called siderophores to compete for iron during iron-limitation. The siderophores isolated from terrestrial bacteria and fungi and from marine bacteria show promise as biomedical agents and for control of microbial growth. To date, no iron-binding compounds produced by marine fungi have been structurally characterized. The siderophores produced by marine fungi provide an as yet unexplored source of novel bioactive compounds. The proposed project will provide the first examples of marine fungal siderophore structures. The specific aims of this project are to: 1. Screen open-ocean fungal strains for the production of iron-binding compounds under conditions of iron limitation. 2. Isolate iron-binding compounds from open-ocean fungal strains. 3. Determine the structure(s) of siderophore(s) produced by open-ocean fungi using amino acid analysis, NMR, and mass spectrometry. Involvement of undergraduate students in research is a vital component of their training and education. The proposed research will be accomplished in collaboration with undergraduate students from Northeastern State University. Students will have the opportunity to learn microbiological techniques, natural product isolation and purification techniques including solid phase extraction and reversed-phase HPLC, and structure characterization methods including amino acid analysis, fatty acid analysis, mass spectrometry, and 1D and 2D NMR.