COBRE: U OF KANSAS MEDICAL CTR: CORE B: MOLECULAR BIOLOGY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子生物学核心将为所有Cobre科学家提供技术支持、最先进的设备和必要的分子生物学试剂，以便以经济高效的方式进行需要分子生物学方法的实验。该中心将提供中央DNA测序设施、量化信使核糖核酸表达的实时聚合酶链式反应服务、高通量聚合酶链式反应和基因分型服务，以及用于共享的载体、质粒和细菌的中央银行。核心将为基本设备提供一个中心位置，包括一台ABI Prism 3100遗传分析仪(在当前应用中需要)、一台实时聚合酶链式反应机器、一台Biomek FX分析工作站(液体分配器)和5台热循环仪，以及其他常用的分子生物学设备，如具有密度分析能力的成像系统、细菌培养箱和超速离心机。Core的工作人员是经验丰富的分子生物学家，他们不仅在使用这种设备方面有实践经验，而且在管理核心设施方面也有经过证明的记录。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core will provide all COBRE scientists with technical support, state-of-the-art equipment, and essential molecular biology reagents, so that experiments which require a molecular biology approach can be carried out in a cost-effective way. The Core will provide a central DNA-sequencing facility, real-time PCR services to quantify mRNA expression, high-throughput PCR and genotyping services, and a central bank of vectors, plasmids, and bacteria for sharing. The Core will provide a central location for essential equipment, including an ABI Prism 3100 Genetic Analyzer (requested in the current application), a real-time PCR machine, a Biomek FX Assay Work Station (liquid dispenser), and 5 thermocyclers, and other commonly used molecular biology equipment, such as an imaging system with the capability for densitometry analysis, bacteria incubators, and ultracentrifuges. The Core is staffed with experienced molecular biologists, who not only have hands-on experience in using this equipment, but also a proven track record in managing a core facility.