MICROSPHERE BASED DNA ANALYSIS FOR PATHOGEN POINT DETECTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们将继续努力开发基于微球的分析方法，以开发一种新的环境样本基因分析方法，以实现对病原体的敏感和快速检测，并具有高度的置信度和较低的误警率。具体地说，我们将开发基于微球的多重分析试剂盒，以提供对从环境样本中扩增的病原体特异性PCR产物的快速点检测，从而允许在一次测试中筛选一组微生物。由于以流式细胞仪为分析平台，样品制备简单，不需要洗涤步骤，分析速度快，不到一分钟。新的分析方法将与商用实验室流动细胞仪以及较小的现场仪器兼容，包括作为能源部CBNP努力的一部分正在开发的自主病原体检测器(APD)。这些新工具将允许灵活的设计，以便在各种应对方案中提供快速分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will expand our ongoing efforts in the development of microsphere-based assays to develop a new approach to the genetic analysis of environmental samples that will enable sensitive and rapid detection of pathogens with a high degree of confidence and a low false alarm rate. Specifically, we will develop multiplex microsphere-based assay kits to provide rapid point detection of pathogen-specific PCR products amplified from environmental samples, allowing screening for a panel of organisms in a single test. Because flow cytometry is the analysis platform, sample preparation is simple, requiring no wash steps, and analysis is rapid, less than one minute. The new assays will be compatible with commercial laboratory flow cytometers as well as smaller fieldable instruments, including the Autonomous Pathogen Detector (APD) being developed as part of the DOE CBNP effort. These new tools will allow flexible design to provide rapid analysis in a variety of response scenarios.