LIGAND-RECEPTOR SYSTEMS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们已经开始检查用于流式细胞术的珠上展示的受体和信号分子（Nolan和Sklar，1998; Sklar等人2000）。我们已经展示了His标记的受体可以与Ni 2+珠结合并结合配体的条件。我们还表明，可溶性受体可以用异源三聚体G蛋白（来自Neubig的亚基）和抑制蛋白（Prossnitz）重建，用于荧光，并可能用于流式细胞术分析。 我们已经尝试使用抑制蛋白、受体以及生物素化的受体尾肽作为珠上的诱饵。我们正在检查生物素化的抗his抗体，而不是螯合的Ni 2+珠。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have begun to examine both receptors and signaling molecules displayed on beads for flow cytometry (Nolan and Sklar, 1998; Sklar et al. 2000). We have shown conditions under which his-tagged receptors may be bound to Ni2+ beads and bind ligand. We have also shown that solublized receptors may be reconstituted with heterotrimeric G proteins (subunits from Neubig) and arrestins (Prossnitz) for fluorescence, and potentially for flow cytometric analysis. We have attempted to use arrestin, receptors, as well as biotinylated receptor tail peptides as bait on beads. We are examining biotinylated anti-his antibodies instead of chelated Ni2+ beads.