SINGLE PARTICLE RECONSTRUCTION OF VIRAL DNA PUMPS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 门户蛋白是一种大的寡聚体DNA泵，它将拖尾噬菌体的二十面体衣壳连接到病毒尾巴上。尽管门户蛋白的大小和复杂的结构组织，但它是由一个自组装产生十二聚体环的单一多肽链形成的。人们对双链dna泵入噬菌体衣壳和从噬菌体衣壳泵出的分子机制知之甚少，这项工作可能会对这一过程产生实质性的新见解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Portal proteins are large oligomeric DNA pumps, which connect the icosahedral capsid of tailed bacteriophages to the viral tail. Despite the large size and complex structural organization, portal proteins are formed by a single polipeptide chain that self assembles to yield a dodecameric ring. Little is known about the molecular mechanisms of double stranded DNA pumping into and from bacteriophage capsids, and this work could yield substantial new insights into this process