TYPE II SECRETION SYSTEM FROM VIBRIO CHOLERAE AND RELATED PATHOGENS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 革兰氏阴性细菌已经进化出许多蛋白质机器，用于通过细胞被膜输出小分子和整个蛋白质。II型分泌系统（T2 SS）使这些细菌能够通过外膜从周质输出折叠蛋白质。T2 SS由12-15种蛋白质组成，它们跨越了 蛋白质传导 通过一组周质蛋白和胞质中的ATP酶将外膜上的孔连接到内膜亚复合体上。人类病原体霍乱弧菌使用这样的系统输出水解酶和霍乱毒素（CT），霍乱毒素是毁灭性疾病霍乱的主要病原体。在产肠球菌E.大肠杆菌（ETEC）分泌热不稳定肠毒素（LT），CT的同源物。ETEC是导致儿童发展中国家的腹泻、脱水和死亡。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Gram negative bacteria have evolved a number of protein machineries for the export of small molecules and entire proteins through the cell envelope. The Type II Secretion System (T2SS) enables these bacteria to export folded proteins from the periplasm through the outer membrane. The T2SS consists of 12-15 proteins, which span from the protein conducting pore in the outer membrane via a set of periplasmic proteins to the inner membrane subcomplex and a regulating ATPase in the cytosol. The human pathogen Vibrio cholerae uses such a system for the export of hydrolytic enzymes and cholera toxin (CT), the major causative agent of the devastating disease cholera. A similar system is found in Enterotoxigenic E. coli (ETEC) which secretes heat-labile enterotoxin (LT), a close homolog of CT. ETEC is a major cause of childrens diarrhea, dehydration and death in developing countries.