USE OF F2 BEAMLINE TO COLLECT MAD DATA FOR OUTER MEMBRANE PROTEIN PAPC

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 PapC是位于革兰氏阴性菌外膜上的一个引导者，负责致病性P菌毛的组装和分泌。它也是进行所谓的“捐助链交换”的平台。PapC的晶体结构将为理解Usher如何重组菌毛亚基/分子伴侣复合物、如何移位菌毛亚基以及如何组装菌毛提供重要信息，这是分子伴侣-Usher菌毛生物合成途径的基本机制。 我们已经使PapC结晶，并且晶体在家用X射线机中衍射到~3.2埃。晶体属I422型，晶胞尺寸a=B=120，c=350。我们需要波束时间来收集MAD或SAD数据集以确定第一引导器结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. PapC is an usher located in the outer membrane of Gram-negative bacteria, responsible for the assembly and secretion of pathogenic P pilus. It is also a platform for the so-called `donor-strand exchange` taking place. Crystal strucutre of PapC will provide important information for understanding how usher recongizing the pilus subunit/chaperone complex, how to translocate pilus subunits, and how to assemle the pilus --- the basic mechanism of so called chaperone-usher pilus biogenesis pathway. We have crystallized PapC, and the crystals diffracted to ~3.2 angstrom at home x-ray machine. the crystals belong to I422, with cell dimentions: a=b=120, c=350. we need beamtime to collected MAD or SAD datasets to determine the first usher structure.