SYNTHETIC APERTURE TOMOGRAPHY FOR HIGH-THROUGHTPUT LIVE CELLS IMAGING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 层析相位显微镜提供有关活细胞的定量信息和高度详细的结构信息。但以目前的形式，它一次只能检测少量细胞。为了在短时间内对大量细胞进行成像，我们开发了一种新的3D相位显微镜，称为合成孔径层析成像（SAT），它可以并入流式细胞术系统。在SAT中，聚焦光束照射到样品上，并且样品在光束上扫描。在获得作为样本位置的函数的透射聚焦光束的相位和振幅图像之后，合成图像以生成一组角投影相位图像。通过应用滤波反投影算法，我们可以映射的三维分布的折射率在活细胞。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Tomographic phase microscopy provides quantitative information and highly detailed structural information about a live cell. But in its current form, it can examine only a small number of cells at a time. To image large number of cells in short time, we have developed a new 3D phase microscopy, called as synthetic aperture tomography (SAT), which can be incorporated into the flow cytometry system. In SAT, a focused beam is illuminated onto a sample and the sample is scanned across the beam. After obtaining both phase and amplitude images of transmitted focused beam as a function of sample position, images are synthesized to generate a set of angular projection phase images. By applying filtered back-projection algorithm, we could map the 3D distribution of refractive index in live cells.