MICROSENSORS BASED ON ENZYMES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 对于不具有电化学活性的分子的通量测量，我们一直使用酶作为自参比电极。例如，在各种类型的酶中，氧化物酶的使用受到青睐，因为它们在氧气存在下的酶催化氧化产生电化学可检测到的过氧化氢。此外，我们已经生成了使用陶瓷溶胶-凝胶基质进行材料封装的设计简化的初步数据。我们预计，这种设计将在传感器结构中有广泛的应用，对几种电位和安培方法产生影响。使用这种凝胶使我们能够开始使用基于脱氢酶的检测，并取得了一定的成功。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For the flux measurements of molecules that are not electrochemically active, we have been employing enzymes for self-referencing electrodes. For example, among various enzyme types, the use of oxidases is favored as they produce electrochemically detectable hydrogen peroxide as a result of enzymatic oxidation in the presence of oxygen. In addition we have generated preliminary data on a design simplification using ceramic sol-gel matrices for encapsulation of materials. We anticipate that this design will have wide application to sensor construction, impacting on several potentiometric and amperometric approaches. Using such a gel has allowed us to begin using dehydrogenase-based detection with moderate success.