MICROSENSORS BASED ON ENZYMES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 对于不具有电化学活性的分子的通量测量，我们一直采用酶作为自参考电极。例如，在各种酶类型中，使用氧化酶是有利的，因为它们在氧存在下由于酶促氧化而产生电化学可检测的过氧化氢。 此外，我们已经产生了初步的数据，设计简化使用陶瓷溶胶-凝胶基质封装的材料。我们预计，这种设计将有广泛的应用传感器的建设，影响几个电位和电流的方法。使用这种凝胶使我们能够开始使用基于内切酶的检测，并取得了一定的成功。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For the flux measurements of molecules that are not electrochemically active, we have been employing enzymes for self-referencing electrodes. For example, among various enzyme types, the use of oxidases is favored as they produce electrochemically detectable hydrogen peroxide as a result of enzymatic oxidation in the presence of oxygen. In addition we have generated preliminary data on a design simplification using ceramic sol-gel matrices for encapsulation of materials. We anticipate that this design will have wide application to sensor construction, impacting on several potentiometric and amperometric approaches. Using such a gel has allowed us to begin using dehydrogenase-based detection with moderate success.