ULTRA-MICRO OXYGEN SENSOR DEVELOPMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该资源见证了对针对新陈代谢的应用程序的日益增长的需求。为了促进这一点，我们开发了几种新技术或整合了其他技术，以允许通过几种方法研究细胞新陈代谢。其中一些在其他地方也有报道。一项关键技术是应用了迄今为止手工建造的自参照微型传感器(见SPID 0047)。虽然我们在这里的效率有了很大的提高，但手工生产安培传感器仍然是一个瓶颈。为了避免这一点，需要开发某种形式的自动化或半自动生产，提供满足信号检测的非侵入性调制技术的严格要求的微电极供应。 鉴于新陈代谢对疾病病理学的重要性，新型氧传感器的问世将促进大量的生物医学研究，并可能提供一种在单细胞分析水平上针对药物开发的新方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The resource has witnessed an increasing demand for applications targeting metabolism. To facilitate this we have developed several new technologies or integrated others to allow the study of cellular metabolism through several approaches. A number of these are reported on elsewhere. A key technique has been the application of self-referencing microsensors which to date have been constructed by hand (see SPID 0047). Although our efficiency here has greatly improved, manual production of amperometric sensors remains a bottleneck. To circumvent this some form of automated or semi automated production needs to be developed providing a supply of microelectrodes meeting the exacting requirements of a non-invasive modulation technique for signal detection. Given the importance of metabolism to the pathology of diseases the availability of the new oxygen sensor will facilitate numerous biomedical investigations and potentially provide a novel methodology for targeting drug development at the level of single cell assays.