IMAGING LUNG CANCER IN MICE TO IMPROVE RADIATION THERAPY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这是一项探索MicroCT作为肺癌定量标记物的试点项目。作为正在进行的K08拨款的一部分，我们将研究一些治疗方法在这种原发肺癌模型中的疗效。 这一试点项目有几个目标： 具体目标1--该项目将帮助建立支持K08的扩展研究所需的后勤支持。更具体地说，该项目将作为一种工具，为未来的研究制定动物移植和IACUC。这也将提供一种简化门控方案的工具。目前，动物被插管进行扫描 同步换气，心电监护。我们建议使用脉搏血氧计作为一种侵入性较小的方法，以简化采集过程。 具体目标2-之前的工作@MGH在没有心脏和呼吸门控的情况下收集了合理的图像数据。我们的假设是，缺乏这种支持会导致对肿瘤体积的高估。通过比较有无门控的采集情况，我们将具体确定门控对肿瘤体积的影响。这是一个 求生协议。因此，这个项目将成为强调我们生存支持技能的工具。 特殊目的3-组织学证据表明，这些肿瘤的血管密度约为体积的15%。我们假设双重扫描(使用/不使用血池试剂)将使我们能够量化血管密度及其变化。该项目将检验这一假设，并为R01奠定基础 申请。 具体目标4-在这一点上，microPET一直被认为是检测小肿瘤的金标准。我们假设，对于小鼠，microCT的分辨率可能足够高，以提供比当前金标准更高的灵敏度(和特异度)。这也将为计划中的R01应用程序提供支持。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a pilot project to explore the utility of microCT as a quantiative marker for lung cancer. As part of an ongoing K08 grant we will be studying the efficacy of a number of therapies in this model of primary lung cancer. This pilot project has several goals: Specific Aim 1- this project will help establish the logistical support required for the extended studies in support of this K08. More specifically the project will serve as a vehicle to work out animal transfer and IACUC for future studies. This will also provide a vehicle to streamline the gating protocols. Currently animals are intubated for scan synchronous ventilation and monitored with ECG. We propose the use of a pulse oximeter as a less invasive method that will streamline the acquisition. Specific Aim 2- previous work @ MGH has gathered reasonable image data without cardiac and respiratory gating. Our hypothesis is that the absence of this support leads to overestimation of tumor volumes. By comparing the acquisition with and without gating, we will determine specifically the impact of gating on tumor volume. This is a survival protocol. Thus this project will serve as a vehicle to stress our survival support skills. Specific Aim 3 - Histologic evidence suggests that these tumors will have vascular densities of ~ 15% of the volume. We hypothesize that a dual scan (with/without blood pool agent) will allow us to quantiate vascular density and changes in that density. This project will test that hypothesis and lay the ground work for an R01 application. Specific Aim 4 - to this point , microPET has been considered a gold standard for detection of small tumors. We hypothesis that for the mouse, the resolution of the microCT might be sufficiently high to provide an increased level of sensitivity (and specificity) beyond that of the current gold standard. This too will provide support for a planned R01 application.